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Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms

While the major virulence factors for Vibrio cholerae, the cause of the devastating diarrheal disease cholera, have been extensively studied, the initial intestinal colonization of the bacterium is not well understood because non-human adult animals are refractory to its colonization. Recent studies...

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Autores principales: Flaugnatti, Nicolas, Isaac, Sandrine, Lemos Rocha, Leonardo F., Stutzmann, Sandrine, Rendueles, Olaya, Stoudmann, Candice, Vesel, Nina, Garcia-Garcera, Marc, Buffet, Amandine, Sana, Thibault G., Rocha, Eduardo P. C., Blokesch, Melanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486750/
https://www.ncbi.nlm.nih.gov/pubmed/34599171
http://dx.doi.org/10.1038/s41467-021-26041-0
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author Flaugnatti, Nicolas
Isaac, Sandrine
Lemos Rocha, Leonardo F.
Stutzmann, Sandrine
Rendueles, Olaya
Stoudmann, Candice
Vesel, Nina
Garcia-Garcera, Marc
Buffet, Amandine
Sana, Thibault G.
Rocha, Eduardo P. C.
Blokesch, Melanie
author_facet Flaugnatti, Nicolas
Isaac, Sandrine
Lemos Rocha, Leonardo F.
Stutzmann, Sandrine
Rendueles, Olaya
Stoudmann, Candice
Vesel, Nina
Garcia-Garcera, Marc
Buffet, Amandine
Sana, Thibault G.
Rocha, Eduardo P. C.
Blokesch, Melanie
author_sort Flaugnatti, Nicolas
collection PubMed
description While the major virulence factors for Vibrio cholerae, the cause of the devastating diarrheal disease cholera, have been extensively studied, the initial intestinal colonization of the bacterium is not well understood because non-human adult animals are refractory to its colonization. Recent studies suggest the involvement of an interbacterial killing device known as the type VI secretion system (T6SS). Here, we tested the T6SS-dependent interaction of V. cholerae with a selection of human gut commensal isolates. We show that the pathogen efficiently depleted representative genera of the Proteobacteria in vitro, while members of the Enterobacter cloacae complex and several Klebsiella species remained unaffected. We demonstrate that this resistance against T6SS assaults was mediated by the production of superior T6SS machinery or a barrier exerted by group I capsules. Collectively, our data provide new insights into immunity protein-independent T6SS resistance employed by the human microbiota and colonization resistance in general.
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spelling pubmed-84867502021-10-22 Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms Flaugnatti, Nicolas Isaac, Sandrine Lemos Rocha, Leonardo F. Stutzmann, Sandrine Rendueles, Olaya Stoudmann, Candice Vesel, Nina Garcia-Garcera, Marc Buffet, Amandine Sana, Thibault G. Rocha, Eduardo P. C. Blokesch, Melanie Nat Commun Article While the major virulence factors for Vibrio cholerae, the cause of the devastating diarrheal disease cholera, have been extensively studied, the initial intestinal colonization of the bacterium is not well understood because non-human adult animals are refractory to its colonization. Recent studies suggest the involvement of an interbacterial killing device known as the type VI secretion system (T6SS). Here, we tested the T6SS-dependent interaction of V. cholerae with a selection of human gut commensal isolates. We show that the pathogen efficiently depleted representative genera of the Proteobacteria in vitro, while members of the Enterobacter cloacae complex and several Klebsiella species remained unaffected. We demonstrate that this resistance against T6SS assaults was mediated by the production of superior T6SS machinery or a barrier exerted by group I capsules. Collectively, our data provide new insights into immunity protein-independent T6SS resistance employed by the human microbiota and colonization resistance in general. Nature Publishing Group UK 2021-10-01 /pmc/articles/PMC8486750/ /pubmed/34599171 http://dx.doi.org/10.1038/s41467-021-26041-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Flaugnatti, Nicolas
Isaac, Sandrine
Lemos Rocha, Leonardo F.
Stutzmann, Sandrine
Rendueles, Olaya
Stoudmann, Candice
Vesel, Nina
Garcia-Garcera, Marc
Buffet, Amandine
Sana, Thibault G.
Rocha, Eduardo P. C.
Blokesch, Melanie
Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title_full Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title_fullStr Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title_full_unstemmed Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title_short Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms
title_sort human commensal gut proteobacteria withstand type vi secretion attacks through immunity protein-independent mechanisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486750/
https://www.ncbi.nlm.nih.gov/pubmed/34599171
http://dx.doi.org/10.1038/s41467-021-26041-0
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