Cargando…

Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

Patients with COVID-19 shed SARS-CoV-2 RNA in stool, sometimes well after their respiratory infection has cleared. This may be significant for patient health, epidemiology, and diagnosis. However, methods to preserve stool, and to extract and quantify viral RNA are not standardized. We test the perf...

Descripción completa

Detalles Bibliográficos
Autores principales:	Natarajan, Aravind, Han, Alvin, Zlitni, Soumaya, Brooks, Erin F., Vance, Summer E., Wolfe, Marlene, Singh, Upinder, Jagannathan, Prasanna, Pinsky, Benjamin A., Boehm, Alexandria, Bhatt, Ami S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486790/ https://www.ncbi.nlm.nih.gov/pubmed/34599164 http://dx.doi.org/10.1038/s41467-021-25576-6

Ejemplares similares

Standardized and optimized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
por: Natarajan, Aravind, et al.
Publicado: (2021)

Publisher Correction: Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA
por: Natarajan, Aravind, et al.
Publicado: (2021)

Gastrointestinal symptoms and fecal shedding of SARS-CoV-2 RNA suggest prolonged gastrointestinal infection
por: Natarajan, Aravind, et al.
Publicado: (2022)

Tomato brown rugose fruit virus Mo gene is a novel microbial source tracking marker
por: Natarajan, Aravind, et al.
Publicado: (2023)

The Tomato Brown Rugose Fruit Virus Movement Protein Gene Is a Novel Microbial Source Tracking Marker
por: Natarajan, Aravind, et al.
Publicado: (2023)

Bugs as Drugs: Therapeutic Microbes for the Prevention and Treatment of Disease
por: Zlitni, Soumaya, et al.
Publicado: (2019)

Ruminant Fecal Contamination of Drinking Water Introduced Post-Collection in Rural Kenyan Households
por: Hamzah, Latifah, et al.
Publicado: (2020)

Microbes and microbiomes in 2020 and beyond
por: Natarajan, Aravind, et al.
Publicado: (2020)

Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
por: Simpson, Adrian, et al.
Publicado: (2021)

Stool Screening for Sars-Cov-2 RNA for COVID-19 in Patients Undergoing Hematopoietic Cell Transplantation, Cellular Therapy, and Induction Chemotherapy
por: Severyn, Christopher J., et al.
Publicado: (2023)

Enveloped and non-enveloped virus survival on microfiber towels
por: Anderson, Claire E., et al.
Publicado: (2023)

High-frequency fecal indicator bacteria (FIB) observations to assess water quality drivers at an enclosed beach
por: Searcy, Ryan T., et al.
Publicado: (2023)

Divergence of wastewater SARS-CoV-2 and reported laboratory-confirmed COVID-19 incident case data coincident with wide-spread availability of at-home COVID-19 antigen tests
por: Boehm, Alexandria B., et al.
Publicado: (2023)

The gut microbiome: a missing link in understanding the gastrointestinal manifestations of COVID-19?
por: Brooks, Erin F., et al.
Publicado: (2021)

Investigating the Efficacy of Various Handwashing Methods against Enveloped and Non-Enveloped Viruses
por: E. Anderson, Claire, et al.
Publicado: (2023)

CrAssphage for fecal source tracking in Chile: Covariation with norovirus, HF183, and bacterial indicators
por: Jennings, Wiley C., et al.
Publicado: (2020)

More than a Tripledemic: Influenza A Virus, Respiratory Syncytial Virus, SARS-CoV-2, and Human Metapneumovirus in Wastewater during Winter 2022–2023
por: Boehm, Alexandria B., et al.
Publicado: (2023)

Strain-resolved microbiome sequencing reveals mobile elements that drive bacterial competition on a clinical timescale
por: Zlitni, Soumaya, et al.
Publicado: (2020)

Regional Replacement of SARS-CoV-2 Variant Omicron BA.1 with BA.2 as Observed through Wastewater Surveillance
por: Boehm, Alexandria B., et al.
Publicado: (2022)

Fecal indicator bacteria and virus removal in stormwater biofilters: Effects of biochar, media saturation, and field conditioning
por: Kranner, Benjamin P., et al.
Publicado: (2019)

High-Frequency, High-Throughput Quantification of SARS-CoV-2 RNA in Wastewater Settled Solids at Eight Publicly Owned Treatment Works in Northern California Shows Strong Association with COVID-19 Incidence
por: Wolfe, Marlene K., et al.
Publicado: (2021)

Label-Free Quantification (LFQ) of Fecal Proteins for Potential Pregnancy Detection in Polar Bears
por: Curry, Erin, et al.
Publicado: (2022)

SARS-CoV-2 RNA Wastewater Settled Solids Surveillance Frequency and Impact on Predicted COVID-19 Incidence Using a Distributed Lag Model
por: Schoen, Mary E., et al.
Publicado: (2022)

Detection of SARS-CoV-2 Variants Mu, Beta, Gamma, Lambda, Delta, Alpha, and Omicron in Wastewater Settled Solids Using Mutation-Specific Assays Is Associated with Regional Detection of Variants in Clinical Samples
por: Wolfe, Marlene, et al.
Publicado: (2022)

Wastewater concentrations of human influenza, metapneumovirus, parainfluenza, respiratory syncytial virus, rhinovirus, and seasonal coronavirus nucleic-acids during the COVID-19 pandemic: a surveillance study
por: Boehm, Alexandria B, et al.
Publicado: (2023)

Interferon-γ Release Assay for Accurate Detection of Severe Acute Respiratory Syndrome Coronavirus 2 T-Cell Response
por: Murugesan, Kanagavel, et al.
Publicado: (2020)

1330. Clinical Associations and Trajectory of “Long COVID”
por: Jacobson, Karen, et al.
Publicado: (2021)

Application of molecular source tracking and mass balance approach to identify potential sources of fecal indicator bacteria in a tropical river
por: Yamahara, Kevan M., et al.
Publicado: (2020)

Fecal Near Infrared Spectroscopy to Discriminate Physiological Status in Giant Pandas
por: Wiedower, Erin E., et al.
Publicado: (2012)

Effects of preservation method on canine (Canis lupus familiaris) fecal microbiota
por: Horng, Katti R., et al.
Publicado: (2018)

Standards for Collection, Preservation, and Transportation of Fecal Samples in TCM Clinical Trials
por: Su, Wenquan, et al.
Publicado: (2022)

Peginterferon Lambda-1a for treatment of outpatients with uncomplicated COVID-19: a randomized placebo-controlled trial
por: Jagannathan, Prasanna, et al.
Publicado: (2021)

Systematic review of marine environmental DNA metabarcoding studies: toward best practices for data usability and accessibility
por: Shea, Meghan M., et al.
Publicado: (2023)

Precise protein quantification based on peptide quantification using iTRAQ™
por: Boehm, Andreas M, et al.
Publicado: (2007)

Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies
por: Song, Se Jin, et al.
Publicado: (2016)

Long term but not short term exposure to obesity related microbiota promotes host insulin resistance
por: Foley, Kevin P., et al.
Publicado: (2018)

How does malaria in pregnancy impact malaria risk in infants?
por: Jagannathan, Prasanna
Publicado: (2018)

Inflammatory but not respiratory symptoms are associated with ongoing upper airway viral shedding in outpatients with uncomplicated COVID-19
por: Jacobson, Karen B., et al.
Publicado: (2022)

Quantification of Human and Animal Viruses to Differentiate the Origin of the Fecal Contamination Present in Environmental Samples
por: Bofill-Mas, Sílvia, et al.
Publicado: (2013)

Quantification of Methylisothiazolinone and Methylchloroisothiazolinone Preservatives by High-Performance Liquid Chromatography
por: Alanazi, Samyah, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_upkn0ks7ou7juslrmtt1ibopio