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Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma

Circular RNAs (circRNAs), continuous loops of single-stranded RNA, regulate gene expression during the development of various cancers. However, the function of circRNAs in hepatocellular carcinoma (HCC) is rarely discussed. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to deter...

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Autores principales: Ni, Chuangye, Yang, Shikun, Ji, Yang, Duan, Yunfei, Yang, Wenjie, Yang, Xinchen, Li, Min, Xie, Jun, Zhang, Chuanyong, Lu, Yunjie, Lu, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486831/
https://www.ncbi.nlm.nih.gov/pubmed/34599150
http://dx.doi.org/10.1038/s41420-021-00664-0
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author Ni, Chuangye
Yang, Shikun
Ji, Yang
Duan, Yunfei
Yang, Wenjie
Yang, Xinchen
Li, Min
Xie, Jun
Zhang, Chuanyong
Lu, Yunjie
Lu, Hao
author_facet Ni, Chuangye
Yang, Shikun
Ji, Yang
Duan, Yunfei
Yang, Wenjie
Yang, Xinchen
Li, Min
Xie, Jun
Zhang, Chuanyong
Lu, Yunjie
Lu, Hao
author_sort Ni, Chuangye
collection PubMed
description Circular RNAs (circRNAs), continuous loops of single-stranded RNA, regulate gene expression during the development of various cancers. However, the function of circRNAs in hepatocellular carcinoma (HCC) is rarely discussed. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the mRNA levels of circ_0011385, miR-361-3p, and STC2 in 96 pairs of HCC tissues (tumor tissues and adjacent normal tissues), HCC cell lines, and L02 (human normal liver cell line) cells. The relationships between circ_0011385 expression and clinical features of HCC were evaluated. Functional experiments in vitro or in vivo were used to evaluate the biological function of circ_0011385. Bioinformatics analysis was performed to predict miRNAs and mRNAs sponged by circ_0011385. RNA immunoprecipitation (RIP) and dual-luciferase reporter gene assays were used to elucidate the interactions among circ_0011385, miR-361-3p, and STC2 (stanniocalcin 2). ChIP and dual-luciferase reporter gene assays were used to identify the upstream regulator of circ_0011385. High expression of circ_0011385 was observed in HCC tissues and cell lines and was significantly associated with tumor size, TNM stage, and prognosis. In addition, inhibition of circ_0011385 expression prevented the proliferation of HCC cells in vitro and in vivo. Circ_0011385 sponged miR-361-3p, thereby regulating the mRNA expression of STC2. In addition, the transcription of circ_0011385 was regulated by SP3. Circ_0011385 knockdown suppressed cell proliferation and tumor activity in HCC. Circ_0011385 may therefore serve as a new biomarker in the diagnosis and treatment of HCC.
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spelling pubmed-84868312021-10-07 Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma Ni, Chuangye Yang, Shikun Ji, Yang Duan, Yunfei Yang, Wenjie Yang, Xinchen Li, Min Xie, Jun Zhang, Chuanyong Lu, Yunjie Lu, Hao Cell Death Discov Article Circular RNAs (circRNAs), continuous loops of single-stranded RNA, regulate gene expression during the development of various cancers. However, the function of circRNAs in hepatocellular carcinoma (HCC) is rarely discussed. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the mRNA levels of circ_0011385, miR-361-3p, and STC2 in 96 pairs of HCC tissues (tumor tissues and adjacent normal tissues), HCC cell lines, and L02 (human normal liver cell line) cells. The relationships between circ_0011385 expression and clinical features of HCC were evaluated. Functional experiments in vitro or in vivo were used to evaluate the biological function of circ_0011385. Bioinformatics analysis was performed to predict miRNAs and mRNAs sponged by circ_0011385. RNA immunoprecipitation (RIP) and dual-luciferase reporter gene assays were used to elucidate the interactions among circ_0011385, miR-361-3p, and STC2 (stanniocalcin 2). ChIP and dual-luciferase reporter gene assays were used to identify the upstream regulator of circ_0011385. High expression of circ_0011385 was observed in HCC tissues and cell lines and was significantly associated with tumor size, TNM stage, and prognosis. In addition, inhibition of circ_0011385 expression prevented the proliferation of HCC cells in vitro and in vivo. Circ_0011385 sponged miR-361-3p, thereby regulating the mRNA expression of STC2. In addition, the transcription of circ_0011385 was regulated by SP3. Circ_0011385 knockdown suppressed cell proliferation and tumor activity in HCC. Circ_0011385 may therefore serve as a new biomarker in the diagnosis and treatment of HCC. Nature Publishing Group UK 2021-10-01 /pmc/articles/PMC8486831/ /pubmed/34599150 http://dx.doi.org/10.1038/s41420-021-00664-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ni, Chuangye
Yang, Shikun
Ji, Yang
Duan, Yunfei
Yang, Wenjie
Yang, Xinchen
Li, Min
Xie, Jun
Zhang, Chuanyong
Lu, Yunjie
Lu, Hao
Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title_full Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title_fullStr Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title_full_unstemmed Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title_short Hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
title_sort hsa_circ_0011385 knockdown represses cell proliferation in hepatocellular carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486831/
https://www.ncbi.nlm.nih.gov/pubmed/34599150
http://dx.doi.org/10.1038/s41420-021-00664-0
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