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Establishment of porcine and monkey colonic organoids for drug toxicity study

Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoi...

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Autores principales: Li, Haonan, Wang, Yalong, Zhang, Mengxian, Wang, Hong, Cui, Along, Zhao, Jianguo, Ji, Weizhi, Chen, Ye-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486901/
https://www.ncbi.nlm.nih.gov/pubmed/34599392
http://dx.doi.org/10.1186/s13619-021-00094-4
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author Li, Haonan
Wang, Yalong
Zhang, Mengxian
Wang, Hong
Cui, Along
Zhao, Jianguo
Ji, Weizhi
Chen, Ye-Guang
author_facet Li, Haonan
Wang, Yalong
Zhang, Mengxian
Wang, Hong
Cui, Along
Zhao, Jianguo
Ji, Weizhi
Chen, Ye-Guang
author_sort Li, Haonan
collection PubMed
description Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-021-00094-4.
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spelling pubmed-84869012021-10-08 Establishment of porcine and monkey colonic organoids for drug toxicity study Li, Haonan Wang, Yalong Zhang, Mengxian Wang, Hong Cui, Along Zhao, Jianguo Ji, Weizhi Chen, Ye-Guang Cell Regen Methodology Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-021-00094-4. Springer Singapore 2021-10-02 /pmc/articles/PMC8486901/ /pubmed/34599392 http://dx.doi.org/10.1186/s13619-021-00094-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Methodology
Li, Haonan
Wang, Yalong
Zhang, Mengxian
Wang, Hong
Cui, Along
Zhao, Jianguo
Ji, Weizhi
Chen, Ye-Guang
Establishment of porcine and monkey colonic organoids for drug toxicity study
title Establishment of porcine and monkey colonic organoids for drug toxicity study
title_full Establishment of porcine and monkey colonic organoids for drug toxicity study
title_fullStr Establishment of porcine and monkey colonic organoids for drug toxicity study
title_full_unstemmed Establishment of porcine and monkey colonic organoids for drug toxicity study
title_short Establishment of porcine and monkey colonic organoids for drug toxicity study
title_sort establishment of porcine and monkey colonic organoids for drug toxicity study
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8486901/
https://www.ncbi.nlm.nih.gov/pubmed/34599392
http://dx.doi.org/10.1186/s13619-021-00094-4
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