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Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates

PURPOSE: The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. METHODS: Five kinds of media with different glucose and nitrogen concentrations were prepared as...

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Autores principales: Jing, Dalan, Li, Yingyu, Liu, Ziyuan, Huang, Chen, Zhang, Pei, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487384/
https://www.ncbi.nlm.nih.gov/pubmed/34608418
http://dx.doi.org/10.1155/2021/6666303
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author Jing, Dalan
Li, Yingyu
Liu, Ziyuan
Huang, Chen
Zhang, Pei
Wang, Wei
author_facet Jing, Dalan
Li, Yingyu
Liu, Ziyuan
Huang, Chen
Zhang, Pei
Wang, Wei
author_sort Jing, Dalan
collection PubMed
description PURPOSE: The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. METHODS: Five kinds of media with different glucose and nitrogen concentrations were prepared as the liquid and solid forms. The clinical isolates were as follows: 2 Fusarium solani strains. The clinical corneal isolates and the standard strains were inoculated in the solid and liquid media. They were all incubated at 296 for 96 h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media. Morphologic changes of colonies in the solid media were determined under the light microscope. RESULTS: The clinical isolates of Fusarium solani showed stronger reproductive capacity in the abominable nutritional condition. Besides, when the glucose concentration in the medium was consistent with the glucose concentration of aqueous in diabetic patients, the clinical isolates would show the biological features of quicker growth rate and stronger reproductive capacity. CONCLUSIONS: Nitrogen source is essential for fungus reproduction. The clinical isolates showed stronger environmental adaptability under different nutritional conditions and more sensitive to environmental changes.
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spelling pubmed-84873842021-10-03 Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates Jing, Dalan Li, Yingyu Liu, Ziyuan Huang, Chen Zhang, Pei Wang, Wei J Ophthalmol Research Article PURPOSE: The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. METHODS: Five kinds of media with different glucose and nitrogen concentrations were prepared as the liquid and solid forms. The clinical isolates were as follows: 2 Fusarium solani strains. The clinical corneal isolates and the standard strains were inoculated in the solid and liquid media. They were all incubated at 296 for 96 h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media. Morphologic changes of colonies in the solid media were determined under the light microscope. RESULTS: The clinical isolates of Fusarium solani showed stronger reproductive capacity in the abominable nutritional condition. Besides, when the glucose concentration in the medium was consistent with the glucose concentration of aqueous in diabetic patients, the clinical isolates would show the biological features of quicker growth rate and stronger reproductive capacity. CONCLUSIONS: Nitrogen source is essential for fungus reproduction. The clinical isolates showed stronger environmental adaptability under different nutritional conditions and more sensitive to environmental changes. Hindawi 2021-09-23 /pmc/articles/PMC8487384/ /pubmed/34608418 http://dx.doi.org/10.1155/2021/6666303 Text en Copyright © 2021 Dalan Jing et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jing, Dalan
Li, Yingyu
Liu, Ziyuan
Huang, Chen
Zhang, Pei
Wang, Wei
Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title_full Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title_fullStr Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title_full_unstemmed Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title_short Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates
title_sort study of the effect of nutrient environment on the biological characteristics of fusarium solani clinical corneal isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487384/
https://www.ncbi.nlm.nih.gov/pubmed/34608418
http://dx.doi.org/10.1155/2021/6666303
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