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Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC

BACKGROUND: Cisplatin‐based chemotherapy is a therapeutic strategy against non‐small cell lung cancer (NSCLC). However, cancers relapse after chemotherapy due to a dormant state of residual cancer cells. Extracellular vesicles and particles (EVPs) are active carriers of proteins and nucleic acid. He...

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Autores principales: Xu, Jiaqi, Wang, Lujuan, Yin, Na, Chen, Anqi, Yi, Junqi, Tang, Jingqun, Xiang, Juanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487815/
https://www.ncbi.nlm.nih.gov/pubmed/34520129
http://dx.doi.org/10.1111/1759-7714.14147
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author Xu, Jiaqi
Wang, Lujuan
Yin, Na
Chen, Anqi
Yi, Junqi
Tang, Jingqun
Xiang, Juanjuan
author_facet Xu, Jiaqi
Wang, Lujuan
Yin, Na
Chen, Anqi
Yi, Junqi
Tang, Jingqun
Xiang, Juanjuan
author_sort Xu, Jiaqi
collection PubMed
description BACKGROUND: Cisplatin‐based chemotherapy is a therapeutic strategy against non‐small cell lung cancer (NSCLC). However, cancers relapse after chemotherapy due to a dormant state of residual cancer cells. Extracellular vesicles and particles (EVPs) are active carriers of proteins and nucleic acid. Here, we aimed to study the molecular alterations and proteomic characteristics of EPV in dormant and reactivated cancer cells induced by cisplatin. METHODS: We used a short‐term single dose of cisplatin to induce the dormant and reactivated cell status. We examined the gene expressional profiling and proteomic profiling of EVPs from dormant and reactivated cancer cells by RNA‐sequencing and LC–MS/MS. RESULTS: We found substantial changes in gene expression and protein level in EVP. The genes with higher expression in dormant cancer cells were lipid transporter‐ and lipid metabolic‐related genes. A total of 111 EVP proteins were upregulated in dormant cancer cells compared to those in control cells. Fifty differential expressed proteins (DEPs) were identified in EVPs from reactivated cancer cells compared to those in dormant cancer cells. Among the DEPs, we found that apolipoproteins such as APOA1 and APOE were significantly increased in dormant cancer cell‐derived EVPs. Integration of EVP proteomes with transcriptional profiles of cancer cells revealed that the proteomic profiling of EVP derived from cancer cells can reflect the cellular status of cancer cells, which showed an activated lipid metabolism in dormant state. CONCLUSION: Lipoproteins enriched in EVPs reflect the activated lipid metabolism in dormant cancer cells and may provide potential biomarkers or therapeutic targets for cisplatin‐based therapy.
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spelling pubmed-84878152021-10-08 Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC Xu, Jiaqi Wang, Lujuan Yin, Na Chen, Anqi Yi, Junqi Tang, Jingqun Xiang, Juanjuan Thorac Cancer Original Articles BACKGROUND: Cisplatin‐based chemotherapy is a therapeutic strategy against non‐small cell lung cancer (NSCLC). However, cancers relapse after chemotherapy due to a dormant state of residual cancer cells. Extracellular vesicles and particles (EVPs) are active carriers of proteins and nucleic acid. Here, we aimed to study the molecular alterations and proteomic characteristics of EPV in dormant and reactivated cancer cells induced by cisplatin. METHODS: We used a short‐term single dose of cisplatin to induce the dormant and reactivated cell status. We examined the gene expressional profiling and proteomic profiling of EVPs from dormant and reactivated cancer cells by RNA‐sequencing and LC–MS/MS. RESULTS: We found substantial changes in gene expression and protein level in EVP. The genes with higher expression in dormant cancer cells were lipid transporter‐ and lipid metabolic‐related genes. A total of 111 EVP proteins were upregulated in dormant cancer cells compared to those in control cells. Fifty differential expressed proteins (DEPs) were identified in EVPs from reactivated cancer cells compared to those in dormant cancer cells. Among the DEPs, we found that apolipoproteins such as APOA1 and APOE were significantly increased in dormant cancer cell‐derived EVPs. Integration of EVP proteomes with transcriptional profiles of cancer cells revealed that the proteomic profiling of EVP derived from cancer cells can reflect the cellular status of cancer cells, which showed an activated lipid metabolism in dormant state. CONCLUSION: Lipoproteins enriched in EVPs reflect the activated lipid metabolism in dormant cancer cells and may provide potential biomarkers or therapeutic targets for cisplatin‐based therapy. John Wiley & Sons Australia, Ltd 2021-09-14 2021-10 /pmc/articles/PMC8487815/ /pubmed/34520129 http://dx.doi.org/10.1111/1759-7714.14147 Text en © 2021 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Xu, Jiaqi
Wang, Lujuan
Yin, Na
Chen, Anqi
Yi, Junqi
Tang, Jingqun
Xiang, Juanjuan
Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title_full Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title_fullStr Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title_full_unstemmed Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title_short Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC
title_sort proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in nsclc
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487815/
https://www.ncbi.nlm.nih.gov/pubmed/34520129
http://dx.doi.org/10.1111/1759-7714.14147
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