A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae

Klebsiella pneumoniae carbapenemase genes (bla(KPC)) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for bla(KPC) genes and investigations into the molecular characteristics of bla(KPC) positive Klebsiella pneumoniae were necessary. In this study,...

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Autores principales: Zhang, Weiwei, Feng, Yanling, Zhao, Hanqing, Yan, Chao, Feng, Junxia, Gan, Lin, Cui, Jinghua, Liu, Shiyu, Zhang, Rui, Du, Shuheng, Li, Nannan, Xu, Wenjian, Han, Juqiang, Li, Rongkuan, Xue, Guanhua, Yuan, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488121/
https://www.ncbi.nlm.nih.gov/pubmed/34616692
http://dx.doi.org/10.3389/fcimb.2021.746325
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author Zhang, Weiwei
Feng, Yanling
Zhao, Hanqing
Yan, Chao
Feng, Junxia
Gan, Lin
Cui, Jinghua
Liu, Shiyu
Zhang, Rui
Du, Shuheng
Li, Nannan
Xu, Wenjian
Han, Juqiang
Li, Rongkuan
Xue, Guanhua
Yuan, Jing
author_facet Zhang, Weiwei
Feng, Yanling
Zhao, Hanqing
Yan, Chao
Feng, Junxia
Gan, Lin
Cui, Jinghua
Liu, Shiyu
Zhang, Rui
Du, Shuheng
Li, Nannan
Xu, Wenjian
Han, Juqiang
Li, Rongkuan
Xue, Guanhua
Yuan, Jing
author_sort Zhang, Weiwei
collection PubMed
description Klebsiella pneumoniae carbapenemase genes (bla(KPC)) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for bla(KPC) genes and investigations into the molecular characteristics of bla(KPC) positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for bla(KPC) was established. This protocol could be completed at 39°C in 15–20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The bla(KPC) RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 bla(KPC) positive isolates were identified from each sample, of which 23 isolates were K. pneumoniae. ST11 with bla(KPC-2) was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of bla(KPC) positive carbapenem-resistant K.pneumoniae poses significant challenges for public health control.
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spelling pubmed-84881212021-10-05 A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae Zhang, Weiwei Feng, Yanling Zhao, Hanqing Yan, Chao Feng, Junxia Gan, Lin Cui, Jinghua Liu, Shiyu Zhang, Rui Du, Shuheng Li, Nannan Xu, Wenjian Han, Juqiang Li, Rongkuan Xue, Guanhua Yuan, Jing Front Cell Infect Microbiol Cellular and Infection Microbiology Klebsiella pneumoniae carbapenemase genes (bla(KPC)) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for bla(KPC) genes and investigations into the molecular characteristics of bla(KPC) positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for bla(KPC) was established. This protocol could be completed at 39°C in 15–20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The bla(KPC) RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 bla(KPC) positive isolates were identified from each sample, of which 23 isolates were K. pneumoniae. ST11 with bla(KPC-2) was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of bla(KPC) positive carbapenem-resistant K.pneumoniae poses significant challenges for public health control. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488121/ /pubmed/34616692 http://dx.doi.org/10.3389/fcimb.2021.746325 Text en Copyright © 2021 Zhang, Feng, Zhao, Yan, Feng, Gan, Cui, Liu, Zhang, Du, Li, Xu, Han, Li, Xue and Yuan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhang, Weiwei
Feng, Yanling
Zhao, Hanqing
Yan, Chao
Feng, Junxia
Gan, Lin
Cui, Jinghua
Liu, Shiyu
Zhang, Rui
Du, Shuheng
Li, Nannan
Xu, Wenjian
Han, Juqiang
Li, Rongkuan
Xue, Guanhua
Yuan, Jing
A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title_full A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title_fullStr A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title_full_unstemmed A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title_short A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae
title_sort recombinase aided amplification assay for rapid detection of the klebsiella pneumoniae carbapenemase gene and its characteristics in klebsiella pneumoniae
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488121/
https://www.ncbi.nlm.nih.gov/pubmed/34616692
http://dx.doi.org/10.3389/fcimb.2021.746325
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