Cargando…

Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas

Vertebrates such as zebrafish have the outstanding ability to fully regenerate their retina upon injury, while mammals, including humans, do not. In zebrafish, upon light-induced injury, photoreceptor regeneration is achieved through reprogramming of Müller glia cells, which proliferate and give ris...

Descripción completa

Detalles Bibliográficos
Autores principales: Lourenço, Raquel, Brandão, Ana S., Borbinha, Jorge, Gorgulho, Rita, Jacinto, António
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488126/
https://www.ncbi.nlm.nih.gov/pubmed/34616723
http://dx.doi.org/10.3389/fcell.2021.667796
_version_ 1784578088665874432
author Lourenço, Raquel
Brandão, Ana S.
Borbinha, Jorge
Gorgulho, Rita
Jacinto, António
author_facet Lourenço, Raquel
Brandão, Ana S.
Borbinha, Jorge
Gorgulho, Rita
Jacinto, António
author_sort Lourenço, Raquel
collection PubMed
description Vertebrates such as zebrafish have the outstanding ability to fully regenerate their retina upon injury, while mammals, including humans, do not. In zebrafish, upon light-induced injury, photoreceptor regeneration is achieved through reprogramming of Müller glia cells, which proliferate and give rise to a self-renewing population of progenitors that migrate to the lesion site to differentiate into the new photoreceptors. The Hippo pathway effector YAP was recently implicated in the response to damage in the retina, but how this transcription coactivator is integrated into the signaling network regulating Müller glia reprogramming has not yet been explored. Here, we show that Yap is required in Müller glia to engage their response to a lesion by regulating their cell cycle reentry and progenitor cell formation, contributing to the differentiation of new photoreceptors. We propose that this regulation is accomplished through a lin28a–ascl1a-dependent mechanism, bona fide Müller glia-reprogramming factors. Overall, this study presents Yap as a key regulator of zebrafish Müller glia reprogramming and consequently retina regeneration upon injury.
format Online
Article
Text
id pubmed-8488126
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84881262021-10-05 Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas Lourenço, Raquel Brandão, Ana S. Borbinha, Jorge Gorgulho, Rita Jacinto, António Front Cell Dev Biol Cell and Developmental Biology Vertebrates such as zebrafish have the outstanding ability to fully regenerate their retina upon injury, while mammals, including humans, do not. In zebrafish, upon light-induced injury, photoreceptor regeneration is achieved through reprogramming of Müller glia cells, which proliferate and give rise to a self-renewing population of progenitors that migrate to the lesion site to differentiate into the new photoreceptors. The Hippo pathway effector YAP was recently implicated in the response to damage in the retina, but how this transcription coactivator is integrated into the signaling network regulating Müller glia reprogramming has not yet been explored. Here, we show that Yap is required in Müller glia to engage their response to a lesion by regulating their cell cycle reentry and progenitor cell formation, contributing to the differentiation of new photoreceptors. We propose that this regulation is accomplished through a lin28a–ascl1a-dependent mechanism, bona fide Müller glia-reprogramming factors. Overall, this study presents Yap as a key regulator of zebrafish Müller glia reprogramming and consequently retina regeneration upon injury. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488126/ /pubmed/34616723 http://dx.doi.org/10.3389/fcell.2021.667796 Text en Copyright © 2021 Lourenço, Brandão, Borbinha, Gorgulho and Jacinto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Lourenço, Raquel
Brandão, Ana S.
Borbinha, Jorge
Gorgulho, Rita
Jacinto, António
Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title_full Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title_fullStr Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title_full_unstemmed Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title_short Yap Regulates Müller Glia Reprogramming in Damaged Zebrafish Retinas
title_sort yap regulates müller glia reprogramming in damaged zebrafish retinas
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488126/
https://www.ncbi.nlm.nih.gov/pubmed/34616723
http://dx.doi.org/10.3389/fcell.2021.667796
work_keys_str_mv AT lourencoraquel yapregulatesmullergliareprogrammingindamagedzebrafishretinas
AT brandaoanas yapregulatesmullergliareprogrammingindamagedzebrafishretinas
AT borbinhajorge yapregulatesmullergliareprogrammingindamagedzebrafishretinas
AT gorgulhorita yapregulatesmullergliareprogrammingindamagedzebrafishretinas
AT jacintoantonio yapregulatesmullergliareprogrammingindamagedzebrafishretinas