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Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate
Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected du...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society for Regenerative Medicine
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488178/ https://www.ncbi.nlm.nih.gov/pubmed/34660855 http://dx.doi.org/10.1016/j.reth.2021.09.002 |
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author | Maekawa, Kyoichiro Natsuda, Koji Hidaka, Masaaki Uematsu, Masafumi Soyama, Akihiko Hara, Takanobu Takatsuki, Mitsuhisa Nagai, Kazuhiro Miura, Kiyonori Eguchi, Susumu |
author_facet | Maekawa, Kyoichiro Natsuda, Koji Hidaka, Masaaki Uematsu, Masafumi Soyama, Akihiko Hara, Takanobu Takatsuki, Mitsuhisa Nagai, Kazuhiro Miura, Kiyonori Eguchi, Susumu |
author_sort | Maekawa, Kyoichiro |
collection | PubMed |
description | Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function. |
format | Online Article Text |
id | pubmed-8488178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Japanese Society for Regenerative Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-84881782021-10-14 Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate Maekawa, Kyoichiro Natsuda, Koji Hidaka, Masaaki Uematsu, Masafumi Soyama, Akihiko Hara, Takanobu Takatsuki, Mitsuhisa Nagai, Kazuhiro Miura, Kiyonori Eguchi, Susumu Regen Ther Original Article Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function. Japanese Society for Regenerative Medicine 2021-09-28 /pmc/articles/PMC8488178/ /pubmed/34660855 http://dx.doi.org/10.1016/j.reth.2021.09.002 Text en © 2021 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Maekawa, Kyoichiro Natsuda, Koji Hidaka, Masaaki Uematsu, Masafumi Soyama, Akihiko Hara, Takanobu Takatsuki, Mitsuhisa Nagai, Kazuhiro Miura, Kiyonori Eguchi, Susumu Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title_full | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title_fullStr | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title_full_unstemmed | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title_short | Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
title_sort | long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488178/ https://www.ncbi.nlm.nih.gov/pubmed/34660855 http://dx.doi.org/10.1016/j.reth.2021.09.002 |
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