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Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor

Galectin-3 (Gal3) is a carbohydrate-binding protein reported to promote angiogenesis by influencing vascular endothelial growth factor-A receptor 2 (VEGFR2) signal transduction. Here we evaluated whether the ability of Gal3 to function as an angiogenic factor involved vascular endothelial growth fac...

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Autores principales: Cano, Issahy, Hu, Zhengping, AbuSamra, Dina B., Saint-Geniez, Magali, Ng, Yin Shan Eric, Argüeso, Pablo, D’Amore, Patricia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488270/
https://www.ncbi.nlm.nih.gov/pubmed/34616740
http://dx.doi.org/10.3389/fcell.2021.734346
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author Cano, Issahy
Hu, Zhengping
AbuSamra, Dina B.
Saint-Geniez, Magali
Ng, Yin Shan Eric
Argüeso, Pablo
D’Amore, Patricia A.
author_facet Cano, Issahy
Hu, Zhengping
AbuSamra, Dina B.
Saint-Geniez, Magali
Ng, Yin Shan Eric
Argüeso, Pablo
D’Amore, Patricia A.
author_sort Cano, Issahy
collection PubMed
description Galectin-3 (Gal3) is a carbohydrate-binding protein reported to promote angiogenesis by influencing vascular endothelial growth factor-A receptor 2 (VEGFR2) signal transduction. Here we evaluated whether the ability of Gal3 to function as an angiogenic factor involved vascular endothelial growth factor (VEGF). To address this possibility we used human retinal microvascular endothelial cells (HRECs) to determine whether exogenous Gal3 requires VEGF to activate VEGFR2 signaling and if Gal3 is required for VEGF to activate VEGFR2. VEGFR2 phosphorylation and HREC migration assays, following either VEGF neutralization with ranibizumab or Gal3 silencing, revealed that VEGF endogenously produced by the HRECs was essential for the effect of exogenous Gal3 on VEGFR2 activation and cell migration, and that VEGF-induced VEGFR2 activation was not dependent on Gal3 in HRECs. Gal3 depletion led to no reduction in VEGF-induced cell function. Since Gal3 has been suggested to be a potential therapeutic target for VEGFR2-mediated angiogenesis, it is crucial to define the possible Gal3-mediated VEGFR2 signal transduction mechanism to aid the development of efficacious therapeutic strategies.
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spelling pubmed-84882702021-10-05 Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor Cano, Issahy Hu, Zhengping AbuSamra, Dina B. Saint-Geniez, Magali Ng, Yin Shan Eric Argüeso, Pablo D’Amore, Patricia A. Front Cell Dev Biol Cell and Developmental Biology Galectin-3 (Gal3) is a carbohydrate-binding protein reported to promote angiogenesis by influencing vascular endothelial growth factor-A receptor 2 (VEGFR2) signal transduction. Here we evaluated whether the ability of Gal3 to function as an angiogenic factor involved vascular endothelial growth factor (VEGF). To address this possibility we used human retinal microvascular endothelial cells (HRECs) to determine whether exogenous Gal3 requires VEGF to activate VEGFR2 signaling and if Gal3 is required for VEGF to activate VEGFR2. VEGFR2 phosphorylation and HREC migration assays, following either VEGF neutralization with ranibizumab or Gal3 silencing, revealed that VEGF endogenously produced by the HRECs was essential for the effect of exogenous Gal3 on VEGFR2 activation and cell migration, and that VEGF-induced VEGFR2 activation was not dependent on Gal3 in HRECs. Gal3 depletion led to no reduction in VEGF-induced cell function. Since Gal3 has been suggested to be a potential therapeutic target for VEGFR2-mediated angiogenesis, it is crucial to define the possible Gal3-mediated VEGFR2 signal transduction mechanism to aid the development of efficacious therapeutic strategies. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488270/ /pubmed/34616740 http://dx.doi.org/10.3389/fcell.2021.734346 Text en Copyright © 2021 Cano, Hu, AbuSamra, Saint-Geniez, Ng, Argüeso and D’Amore. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Cano, Issahy
Hu, Zhengping
AbuSamra, Dina B.
Saint-Geniez, Magali
Ng, Yin Shan Eric
Argüeso, Pablo
D’Amore, Patricia A.
Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title_full Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title_fullStr Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title_full_unstemmed Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title_short Galectin-3 Enhances Vascular Endothelial Growth Factor-A Receptor 2 Activity in the Presence of Vascular Endothelial Growth Factor
title_sort galectin-3 enhances vascular endothelial growth factor-a receptor 2 activity in the presence of vascular endothelial growth factor
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488270/
https://www.ncbi.nlm.nih.gov/pubmed/34616740
http://dx.doi.org/10.3389/fcell.2021.734346
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