Cargando…
MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation
Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases, including hepatocellular carcinoma (HCC). lncRNA TONSL-AS1 has been reported to act as a tumor suppressor in gastric cancer. The present study aimed to investigate the role of TONSL-AS1 in hepatocellular carcin...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488329/ https://www.ncbi.nlm.nih.gov/pubmed/34630715 http://dx.doi.org/10.3892/ol.2021.13069 |
_version_ | 1784578141543464960 |
---|---|
author | Deng, Xuesong Cheng, Jun Zhan, Naiyang Chen, Jianwei Zhan, Yongqiang Ni, Yong Liao, Caixian |
author_facet | Deng, Xuesong Cheng, Jun Zhan, Naiyang Chen, Jianwei Zhan, Yongqiang Ni, Yong Liao, Caixian |
author_sort | Deng, Xuesong |
collection | PubMed |
description | Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases, including hepatocellular carcinoma (HCC). lncRNA TONSL-AS1 has been reported to act as a tumor suppressor in gastric cancer. The present study aimed to investigate the role of TONSL-AS1 in hepatocellular carcinoma (HCC). Reverse transcription-quantitative PCR analysis was performed to detect the expression levels of TONSL-AS1 and microRNA (miRNA/miR)-135a in HCC tissues and paired adjacent normal tissues. A 5-year follow-up study was performed to determine the prognostic value of TONSL-AS1 in HCC. The association between miR-135a and TONSL-AS1 was assessed via overexpression experiments. The Cell Counting Kit-8 assay was performed to assess cell proliferation. The results demonstrated that TONSL-AS1 expression was downregulated in HCC tissues, which was associated with a lower survival rate in patients with HCC. TONSL-AS1 and miR-135a were predicted to interact with each other, whereby overexpression of miR-135a downregulated TONSL-AS1 expression. The results demonstrated that TONSL-AS1 and miR-135a were inversely correlated with each other. Notably, overexpression of TONSL-AS1 inhibited HCC cell proliferation, while overexpression of miR-135a promoted HCC cell proliferation and decreased the effect of overexpression of TONSL-AS1 on cell proliferation. Taken together, the results of the present study suggest that miR-135a expression is upregulated in HCC and targets lncRNA TONSL-AS1 to suppress cell proliferation. |
format | Online Article Text |
id | pubmed-8488329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84883292021-10-07 MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation Deng, Xuesong Cheng, Jun Zhan, Naiyang Chen, Jianwei Zhan, Yongqiang Ni, Yong Liao, Caixian Oncol Lett Articles Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases, including hepatocellular carcinoma (HCC). lncRNA TONSL-AS1 has been reported to act as a tumor suppressor in gastric cancer. The present study aimed to investigate the role of TONSL-AS1 in hepatocellular carcinoma (HCC). Reverse transcription-quantitative PCR analysis was performed to detect the expression levels of TONSL-AS1 and microRNA (miRNA/miR)-135a in HCC tissues and paired adjacent normal tissues. A 5-year follow-up study was performed to determine the prognostic value of TONSL-AS1 in HCC. The association between miR-135a and TONSL-AS1 was assessed via overexpression experiments. The Cell Counting Kit-8 assay was performed to assess cell proliferation. The results demonstrated that TONSL-AS1 expression was downregulated in HCC tissues, which was associated with a lower survival rate in patients with HCC. TONSL-AS1 and miR-135a were predicted to interact with each other, whereby overexpression of miR-135a downregulated TONSL-AS1 expression. The results demonstrated that TONSL-AS1 and miR-135a were inversely correlated with each other. Notably, overexpression of TONSL-AS1 inhibited HCC cell proliferation, while overexpression of miR-135a promoted HCC cell proliferation and decreased the effect of overexpression of TONSL-AS1 on cell proliferation. Taken together, the results of the present study suggest that miR-135a expression is upregulated in HCC and targets lncRNA TONSL-AS1 to suppress cell proliferation. D.A. Spandidos 2021-11 2021-09-24 /pmc/articles/PMC8488329/ /pubmed/34630715 http://dx.doi.org/10.3892/ol.2021.13069 Text en Copyright: © Deng et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Deng, Xuesong Cheng, Jun Zhan, Naiyang Chen, Jianwei Zhan, Yongqiang Ni, Yong Liao, Caixian MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title | MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title_full | MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title_fullStr | MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title_full_unstemmed | MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title_short | MicroRNA-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding RNA TONSL-AS1 to suppress cell proliferation |
title_sort | microrna-135a expression is upregulated in hepatocellular carcinoma and targets long non-coding rna tonsl-as1 to suppress cell proliferation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488329/ https://www.ncbi.nlm.nih.gov/pubmed/34630715 http://dx.doi.org/10.3892/ol.2021.13069 |
work_keys_str_mv | AT dengxuesong microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT chengjun microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT zhannaiyang microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT chenjianwei microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT zhanyongqiang microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT niyong microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation AT liaocaixian microrna135aexpressionisupregulatedinhepatocellularcarcinomaandtargetslongnoncodingrnatonslas1tosuppresscellproliferation |