Cargando…
Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail
Biofuel derived from halophytic biomass is getting attention owing to the concerns of energy versus food crisis. The disadvantages associated with edible bioenergy resources necessitate the need to explore new feedstocks for sustainable biofuel production. In this study, biomass from locally availab...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488365/ https://www.ncbi.nlm.nih.gov/pubmed/34616380 http://dx.doi.org/10.3389/fmicb.2021.714940 |
_version_ | 1784578150511935488 |
---|---|
author | Ansari, Immad Ejaz, Uroosa Abideen, Zainul Gulzar, Salman Syed, Muhammad Noman Liu, Jing Li, Wang Fu, Pengcheng Sohail, Muhammad |
author_facet | Ansari, Immad Ejaz, Uroosa Abideen, Zainul Gulzar, Salman Syed, Muhammad Noman Liu, Jing Li, Wang Fu, Pengcheng Sohail, Muhammad |
author_sort | Ansari, Immad |
collection | PubMed |
description | Biofuel derived from halophytic biomass is getting attention owing to the concerns of energy versus food crisis. The disadvantages associated with edible bioenergy resources necessitate the need to explore new feedstocks for sustainable biofuel production. In this study, biomass from locally available abundant halophytes (Panicum antidotale, Phragmites karka, Halopyrum mucronatum, and Desmostachya bipinnata) was screened for saccharification by an enzyme cocktail composed of cellulase, xylanase, and pectinase from Brevibacillus borstelensis UE10 and UE27, Bacillus aestuarii UE25, Aneurinibacillus thermoaerophilus UE1, and Bacillus vallismortis MH 1. Two types of pretreatment, i.e., with dilute acid and freeze-thaw, were independently applied to the halophytic biomass. Saccharification of acid-pretreated P. karka biomass yielded maximum reducing sugars (9 mg g(–1)) as compared to other plants. Thus, the factors (temperature, pH, substrate concentration, and enzyme units) affecting its saccharification were optimized using central composite design. This statistical model predicted 49.8 mg g(–1) of reducing sugars that was comparable to the experimental value (40 mg g(–1)). Scanning electron microscopy and Fourier-transform infrared spectroscopy showed significant structural changes after pretreatment and saccharification. Therefore, halophytes growing in saline, arid, and semi-arid regions can be promising alternative sources for bioenergy production. |
format | Online Article Text |
id | pubmed-8488365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84883652021-10-05 Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail Ansari, Immad Ejaz, Uroosa Abideen, Zainul Gulzar, Salman Syed, Muhammad Noman Liu, Jing Li, Wang Fu, Pengcheng Sohail, Muhammad Front Microbiol Microbiology Biofuel derived from halophytic biomass is getting attention owing to the concerns of energy versus food crisis. The disadvantages associated with edible bioenergy resources necessitate the need to explore new feedstocks for sustainable biofuel production. In this study, biomass from locally available abundant halophytes (Panicum antidotale, Phragmites karka, Halopyrum mucronatum, and Desmostachya bipinnata) was screened for saccharification by an enzyme cocktail composed of cellulase, xylanase, and pectinase from Brevibacillus borstelensis UE10 and UE27, Bacillus aestuarii UE25, Aneurinibacillus thermoaerophilus UE1, and Bacillus vallismortis MH 1. Two types of pretreatment, i.e., with dilute acid and freeze-thaw, were independently applied to the halophytic biomass. Saccharification of acid-pretreated P. karka biomass yielded maximum reducing sugars (9 mg g(–1)) as compared to other plants. Thus, the factors (temperature, pH, substrate concentration, and enzyme units) affecting its saccharification were optimized using central composite design. This statistical model predicted 49.8 mg g(–1) of reducing sugars that was comparable to the experimental value (40 mg g(–1)). Scanning electron microscopy and Fourier-transform infrared spectroscopy showed significant structural changes after pretreatment and saccharification. Therefore, halophytes growing in saline, arid, and semi-arid regions can be promising alternative sources for bioenergy production. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488365/ /pubmed/34616380 http://dx.doi.org/10.3389/fmicb.2021.714940 Text en Copyright © 2021 Ansari, Ejaz, Abideen, Gulzar, Syed, Liu, Li, Fu and Sohail. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ansari, Immad Ejaz, Uroosa Abideen, Zainul Gulzar, Salman Syed, Muhammad Noman Liu, Jing Li, Wang Fu, Pengcheng Sohail, Muhammad Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title | Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title_full | Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title_fullStr | Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title_full_unstemmed | Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title_short | Wild Halophytic Phragmites karka Biomass Saccharification by Bacterial Enzyme Cocktail |
title_sort | wild halophytic phragmites karka biomass saccharification by bacterial enzyme cocktail |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488365/ https://www.ncbi.nlm.nih.gov/pubmed/34616380 http://dx.doi.org/10.3389/fmicb.2021.714940 |
work_keys_str_mv | AT ansariimmad wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT ejazuroosa wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT abideenzainul wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT gulzarsalman wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT syedmuhammadnoman wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT liujing wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT liwang wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT fupengcheng wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail AT sohailmuhammad wildhalophyticphragmiteskarkabiomasssaccharificationbybacterialenzymecocktail |