Cargando…

METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification

OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation in...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Rong-hua, Du, Peng, Gao, Chao-qun, Liu, Xue-rong, Zhang, Jin-an
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488398/
https://www.ncbi.nlm.nih.gov/pubmed/34616359
http://dx.doi.org/10.3389/fendo.2021.666393
_version_ 1784578158377304064
author Song, Rong-hua
Du, Peng
Gao, Chao-qun
Liu, Xue-rong
Zhang, Jin-an
author_facet Song, Rong-hua
Du, Peng
Gao, Chao-qun
Liu, Xue-rong
Zhang, Jin-an
author_sort Song, Rong-hua
collection PubMed
description OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves’ disease (GD). METHODS: Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4(+) T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells. RESULTS: High throughput microarrays revealed that METTL3 and SOCS molecules were aberrantly expressed in thyroid tissues and CD4(+)T cells of GD compared to the controls. Bioinformatic analysis was undertaken by searching databases of found genes of the SOCS family that possessed many mRNA m6A modification loci. METTL3 knock-down experiment revealed that expressions of SOCS family members SOCS1, SOCS2, SOCS4, SOCS5, and SOCS6 were increased after METTL3 knock-down. CONCLUSIONS: For the first time, the present study revealed the relationship between m6A modification and GD and indicated that METTL3 may be involved in the development of GD by inducing mRNA m6A methylation modification of SOCS family members.
format Online
Article
Text
id pubmed-8488398
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84883982021-10-05 METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification Song, Rong-hua Du, Peng Gao, Chao-qun Liu, Xue-rong Zhang, Jin-an Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves’ disease (GD). METHODS: Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4(+) T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells. RESULTS: High throughput microarrays revealed that METTL3 and SOCS molecules were aberrantly expressed in thyroid tissues and CD4(+)T cells of GD compared to the controls. Bioinformatic analysis was undertaken by searching databases of found genes of the SOCS family that possessed many mRNA m6A modification loci. METTL3 knock-down experiment revealed that expressions of SOCS family members SOCS1, SOCS2, SOCS4, SOCS5, and SOCS6 were increased after METTL3 knock-down. CONCLUSIONS: For the first time, the present study revealed the relationship between m6A modification and GD and indicated that METTL3 may be involved in the development of GD by inducing mRNA m6A methylation modification of SOCS family members. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488398/ /pubmed/34616359 http://dx.doi.org/10.3389/fendo.2021.666393 Text en Copyright © 2021 Song, Du, Gao, Liu and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Song, Rong-hua
Du, Peng
Gao, Chao-qun
Liu, Xue-rong
Zhang, Jin-an
METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title_full METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title_fullStr METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title_full_unstemmed METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title_short METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
title_sort mettl3 is involved in the development of graves’ disease by inducing socs mrna m6a modification
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488398/
https://www.ncbi.nlm.nih.gov/pubmed/34616359
http://dx.doi.org/10.3389/fendo.2021.666393
work_keys_str_mv AT songronghua mettl3isinvolvedinthedevelopmentofgravesdiseasebyinducingsocsmrnam6amodification
AT dupeng mettl3isinvolvedinthedevelopmentofgravesdiseasebyinducingsocsmrnam6amodification
AT gaochaoqun mettl3isinvolvedinthedevelopmentofgravesdiseasebyinducingsocsmrnam6amodification
AT liuxuerong mettl3isinvolvedinthedevelopmentofgravesdiseasebyinducingsocsmrnam6amodification
AT zhangjinan mettl3isinvolvedinthedevelopmentofgravesdiseasebyinducingsocsmrnam6amodification