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METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification
OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488398/ https://www.ncbi.nlm.nih.gov/pubmed/34616359 http://dx.doi.org/10.3389/fendo.2021.666393 |
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author | Song, Rong-hua Du, Peng Gao, Chao-qun Liu, Xue-rong Zhang, Jin-an |
author_facet | Song, Rong-hua Du, Peng Gao, Chao-qun Liu, Xue-rong Zhang, Jin-an |
author_sort | Song, Rong-hua |
collection | PubMed |
description | OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves’ disease (GD). METHODS: Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4(+) T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells. RESULTS: High throughput microarrays revealed that METTL3 and SOCS molecules were aberrantly expressed in thyroid tissues and CD4(+)T cells of GD compared to the controls. Bioinformatic analysis was undertaken by searching databases of found genes of the SOCS family that possessed many mRNA m6A modification loci. METTL3 knock-down experiment revealed that expressions of SOCS family members SOCS1, SOCS2, SOCS4, SOCS5, and SOCS6 were increased after METTL3 knock-down. CONCLUSIONS: For the first time, the present study revealed the relationship between m6A modification and GD and indicated that METTL3 may be involved in the development of GD by inducing mRNA m6A methylation modification of SOCS family members. |
format | Online Article Text |
id | pubmed-8488398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84883982021-10-05 METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification Song, Rong-hua Du, Peng Gao, Chao-qun Liu, Xue-rong Zhang, Jin-an Front Endocrinol (Lausanne) Endocrinology OBJECTIVE: Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves’ disease (GD). METHODS: Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4(+) T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells. RESULTS: High throughput microarrays revealed that METTL3 and SOCS molecules were aberrantly expressed in thyroid tissues and CD4(+)T cells of GD compared to the controls. Bioinformatic analysis was undertaken by searching databases of found genes of the SOCS family that possessed many mRNA m6A modification loci. METTL3 knock-down experiment revealed that expressions of SOCS family members SOCS1, SOCS2, SOCS4, SOCS5, and SOCS6 were increased after METTL3 knock-down. CONCLUSIONS: For the first time, the present study revealed the relationship between m6A modification and GD and indicated that METTL3 may be involved in the development of GD by inducing mRNA m6A methylation modification of SOCS family members. Frontiers Media S.A. 2021-09-20 /pmc/articles/PMC8488398/ /pubmed/34616359 http://dx.doi.org/10.3389/fendo.2021.666393 Text en Copyright © 2021 Song, Du, Gao, Liu and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Song, Rong-hua Du, Peng Gao, Chao-qun Liu, Xue-rong Zhang, Jin-an METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title | METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title_full | METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title_fullStr | METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title_full_unstemmed | METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title_short | METTL3 Is Involved in the Development of Graves’ Disease by Inducing SOCS mRNA m6A Modification |
title_sort | mettl3 is involved in the development of graves’ disease by inducing socs mrna m6a modification |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488398/ https://www.ncbi.nlm.nih.gov/pubmed/34616359 http://dx.doi.org/10.3389/fendo.2021.666393 |
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