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Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy
The number of patients with type 2 diabetes mellitus (T2DM) has increased worldwide. Although an instant cure was achieved with the standard treatment acabose, unsatisfactory symptoms associated with cardiovascular disease after acabose administration have been reported. Therefore, it is important t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488491/ https://www.ncbi.nlm.nih.gov/pubmed/34632145 http://dx.doi.org/10.1016/j.heliyon.2021.e08078 |
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author | Eawsakul, Komgrit Panichayupakaranant, Pharkphoom Ongtanasup, Tassanee Warinhomhoun, Sakan Noonong, Kunwadee Bunluepuech, Kingkan |
author_facet | Eawsakul, Komgrit Panichayupakaranant, Pharkphoom Ongtanasup, Tassanee Warinhomhoun, Sakan Noonong, Kunwadee Bunluepuech, Kingkan |
author_sort | Eawsakul, Komgrit |
collection | PubMed |
description | The number of patients with type 2 diabetes mellitus (T2DM) has increased worldwide. Although an instant cure was achieved with the standard treatment acabose, unsatisfactory symptoms associated with cardiovascular disease after acabose administration have been reported. Therefore, it is important to explore new treatments. A Thai folk recipe has long been used for T2DM treatment, and it effectively decreases blood glucose. However, the mechanism of this recipe has never been proven. Therefore, the potential anti-T2DM effect of this recipe, which is used in Thai hospitals, was determined to involve alpha-glucosidase (AG) inhibition with a half maximal inhibitory concentration (IC(50)). In vitro experiments showed that crude Cinnamomum verum extract (IC(50) = 0.35 ± 0.12 mg/mL) offered excellent inhibitory activity, followed by extracts from Tinospora crispa (IC(50) = 0.69 ± 0.39 mg/mL), Stephania suberosa (IC(50) = 1.50 ± 0.17 mg/mL), Andrographis paniculate (IC(50) = 1.78 ± 0.35 mg/mL), and Thunbergia laurifolia (IC(50) = 4.66 ± 0.27 mg/mL). However, the potencies of these extracts were lower than that of acabose (IC(50) = 0.55 ± 0.11 mg/mL). Therefore, this study investigated and developed a formulation of this recipe using computational docking. Among 61 compounds, 7 effectively inhibited AG, including chlorogenic acid (IC(50) = 819.07 pM) through 5 hydrogen bonds (HBs) and 2 hydrophobic interactions (HIs); β-sitosterol (IC(50) = 4.46 nM, 6 HIs); ergosterol peroxide (IC(50) = 4.18 nM, 6 HIs); borapetoside D (IC(50) = 508.63 pM, 7 HBs and 2 HIs); borapetoside A (IC(50) = 1.09 nM, 2 HBs and 2 His), stephasubimine (IC(50) = 285.37 pM, 6 HIs); and stephasubine (IC(50) = 1.09 nM, 3 HBs and 4 HIs). These compounds bind with high affinity to different binding pockets, leading to additive effects. Moreover, the pharmacokinetics of six of these seven compounds (except ergosterol peroxide) showed poor absorption in the gastrointestinal tract, which would allow for competitive binding to AG in the small intestine. These results indicate that the development of these 6 compounds into oral antidiabetic agents is promising. |
format | Online Article Text |
id | pubmed-8488491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-84884912021-10-08 Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy Eawsakul, Komgrit Panichayupakaranant, Pharkphoom Ongtanasup, Tassanee Warinhomhoun, Sakan Noonong, Kunwadee Bunluepuech, Kingkan Heliyon Research Article The number of patients with type 2 diabetes mellitus (T2DM) has increased worldwide. Although an instant cure was achieved with the standard treatment acabose, unsatisfactory symptoms associated with cardiovascular disease after acabose administration have been reported. Therefore, it is important to explore new treatments. A Thai folk recipe has long been used for T2DM treatment, and it effectively decreases blood glucose. However, the mechanism of this recipe has never been proven. Therefore, the potential anti-T2DM effect of this recipe, which is used in Thai hospitals, was determined to involve alpha-glucosidase (AG) inhibition with a half maximal inhibitory concentration (IC(50)). In vitro experiments showed that crude Cinnamomum verum extract (IC(50) = 0.35 ± 0.12 mg/mL) offered excellent inhibitory activity, followed by extracts from Tinospora crispa (IC(50) = 0.69 ± 0.39 mg/mL), Stephania suberosa (IC(50) = 1.50 ± 0.17 mg/mL), Andrographis paniculate (IC(50) = 1.78 ± 0.35 mg/mL), and Thunbergia laurifolia (IC(50) = 4.66 ± 0.27 mg/mL). However, the potencies of these extracts were lower than that of acabose (IC(50) = 0.55 ± 0.11 mg/mL). Therefore, this study investigated and developed a formulation of this recipe using computational docking. Among 61 compounds, 7 effectively inhibited AG, including chlorogenic acid (IC(50) = 819.07 pM) through 5 hydrogen bonds (HBs) and 2 hydrophobic interactions (HIs); β-sitosterol (IC(50) = 4.46 nM, 6 HIs); ergosterol peroxide (IC(50) = 4.18 nM, 6 HIs); borapetoside D (IC(50) = 508.63 pM, 7 HBs and 2 HIs); borapetoside A (IC(50) = 1.09 nM, 2 HBs and 2 His), stephasubimine (IC(50) = 285.37 pM, 6 HIs); and stephasubine (IC(50) = 1.09 nM, 3 HBs and 4 HIs). These compounds bind with high affinity to different binding pockets, leading to additive effects. Moreover, the pharmacokinetics of six of these seven compounds (except ergosterol peroxide) showed poor absorption in the gastrointestinal tract, which would allow for competitive binding to AG in the small intestine. These results indicate that the development of these 6 compounds into oral antidiabetic agents is promising. Elsevier 2021-09-29 /pmc/articles/PMC8488491/ /pubmed/34632145 http://dx.doi.org/10.1016/j.heliyon.2021.e08078 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Eawsakul, Komgrit Panichayupakaranant, Pharkphoom Ongtanasup, Tassanee Warinhomhoun, Sakan Noonong, Kunwadee Bunluepuech, Kingkan Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title | Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title_full | Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title_fullStr | Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title_full_unstemmed | Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title_short | Computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a Thai folk remedy |
title_sort | computational study and in vitro alpha-glucosidase inhibitory effects of medicinal plants from a thai folk remedy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488491/ https://www.ncbi.nlm.nih.gov/pubmed/34632145 http://dx.doi.org/10.1016/j.heliyon.2021.e08078 |
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