CloneSeq - Single-cell clonal 3D culture and analysis protocol

This CloneSeq protocol combines clonal expansion inside 3D hydrogel spheres and droplet-based RNA sequencing to resolve the limited sensitivity of single-cell approaches. CloneSeq can reveal rare subpopulations and support cellular stemness. CloneSeq can be adapted to different biological systems to...

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Detalles Bibliográficos
Autores principales: Sun, Xue, Bavli, Danny, Kozulin, Chen, Motzik, Alex, Buxboim, Amnon, Ram, Oren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488600/
https://www.ncbi.nlm.nih.gov/pubmed/34632413
http://dx.doi.org/10.1016/j.xpro.2021.100794

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488600/
https://www.ncbi.nlm.nih.gov/pubmed/34632413
http://dx.doi.org/10.1016/j.xpro.2021.100794

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