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CloneSeq - Single-cell clonal 3D culture and analysis protocol

This CloneSeq protocol combines clonal expansion inside 3D hydrogel spheres and droplet-based RNA sequencing to resolve the limited sensitivity of single-cell approaches. CloneSeq can reveal rare subpopulations and support cellular stemness. CloneSeq can be adapted to different biological systems to...

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Detalles Bibliográficos
Autores principales:	Sun, Xue, Bavli, Danny, Kozulin, Chen, Motzik, Alex, Buxboim, Amnon, Ram, Oren
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488600/ https://www.ncbi.nlm.nih.gov/pubmed/34632413 http://dx.doi.org/10.1016/j.xpro.2021.100794

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