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Searching for Hydrodynamic Orienting Effects in the Association of Tri-N-acetylglucosamine with Hen Egg-White Lysozyme
[Image: see text] Using stopped-flow fluorometry, we determined rate constants for the formation of diffusional encounter complexes of tri-N-acetylglucosamine (NAG(3)) with hen egg-white lysozyme (k(a)(WT)) and its double mutant Asp48Asn/Lys116Gln (k(a)(MT)). We defined binding anisotropy, κ ≡ (k(a)...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488934/ https://www.ncbi.nlm.nih.gov/pubmed/34546051 http://dx.doi.org/10.1021/acs.jpcb.1c06762 |