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LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA
Objective: This study aims to determine the expression pattern of long non-coding RNA (lncRNA) TUSC8 in esophageal cancer tissues and cell lines, to investigate its effects on esophageal cancer cell proliferation and migration, and to explore the mechanism of TUSC8-mediated esophageal cancer suppres...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489135/ https://www.ncbi.nlm.nih.gov/pubmed/34659529 http://dx.doi.org/10.7150/jca.57814 |
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author | Hu, Rui Bi, Rui Jiang, Lianyong Yang, Xuhui Zhong, Yuan Xie, Xiao |
author_facet | Hu, Rui Bi, Rui Jiang, Lianyong Yang, Xuhui Zhong, Yuan Xie, Xiao |
author_sort | Hu, Rui |
collection | PubMed |
description | Objective: This study aims to determine the expression pattern of long non-coding RNA (lncRNA) TUSC8 in esophageal cancer tissues and cell lines, to investigate its effects on esophageal cancer cell proliferation and migration, and to explore the mechanism of TUSC8-mediated esophageal cancer suppression via VEGFA downregulation. Patients and Methods: TUSC8 levels in esophageal cancer tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influence of TUSC8 on clinical features in esophageal cancer patients was analyzed. After intervening TUSC8 expression in esophageal cancer cells, the proliferative and migratory abilities were examined in OE19 and TE-1 cells through a series of function experiments. The interaction between TUSC8 and VEGFA was assessed by the bioinformatics prediction and dual-luciferase reporter assay. Finally, the co-regulation of TUSC8 and VEGFA on esophageal cancer cell functions was evaluated. Results: TUSC8 was downregulated in esophageal cancer tissues compared with normal ones. Identically, decreased TUSC8 expression was detected in esophageal cancer cell lines compared with control cells. Low TUSC8 expression predicted poor prognosis in patients with esophageal cancer. Knockdown of TUSC8 promoted the proliferative and migratory abilities in OE19 cells, whereas overexpression of TUSC8 resulted in opposite results in TE-1 cells. VEGFA was confirmed to be a target gene of TUSC8. Overexpression of VEGFA could reverse the regulatory effects of TUSC8 on esophageal cancer cell proliferation and migration. Conclusions: LncRNA TUSC8 is downregulated in esophageal cancer tissues and cell lines. TUSC8 inhibits the proliferative and migratory abilities in esophageal cancer cells in vitro by negatively regulating VEGFA. |
format | Online Article Text |
id | pubmed-8489135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-84891352021-10-15 LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA Hu, Rui Bi, Rui Jiang, Lianyong Yang, Xuhui Zhong, Yuan Xie, Xiao J Cancer Research Paper Objective: This study aims to determine the expression pattern of long non-coding RNA (lncRNA) TUSC8 in esophageal cancer tissues and cell lines, to investigate its effects on esophageal cancer cell proliferation and migration, and to explore the mechanism of TUSC8-mediated esophageal cancer suppression via VEGFA downregulation. Patients and Methods: TUSC8 levels in esophageal cancer tissues and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The influence of TUSC8 on clinical features in esophageal cancer patients was analyzed. After intervening TUSC8 expression in esophageal cancer cells, the proliferative and migratory abilities were examined in OE19 and TE-1 cells through a series of function experiments. The interaction between TUSC8 and VEGFA was assessed by the bioinformatics prediction and dual-luciferase reporter assay. Finally, the co-regulation of TUSC8 and VEGFA on esophageal cancer cell functions was evaluated. Results: TUSC8 was downregulated in esophageal cancer tissues compared with normal ones. Identically, decreased TUSC8 expression was detected in esophageal cancer cell lines compared with control cells. Low TUSC8 expression predicted poor prognosis in patients with esophageal cancer. Knockdown of TUSC8 promoted the proliferative and migratory abilities in OE19 cells, whereas overexpression of TUSC8 resulted in opposite results in TE-1 cells. VEGFA was confirmed to be a target gene of TUSC8. Overexpression of VEGFA could reverse the regulatory effects of TUSC8 on esophageal cancer cell proliferation and migration. Conclusions: LncRNA TUSC8 is downregulated in esophageal cancer tissues and cell lines. TUSC8 inhibits the proliferative and migratory abilities in esophageal cancer cells in vitro by negatively regulating VEGFA. Ivyspring International Publisher 2021-09-03 /pmc/articles/PMC8489135/ /pubmed/34659529 http://dx.doi.org/10.7150/jca.57814 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hu, Rui Bi, Rui Jiang, Lianyong Yang, Xuhui Zhong, Yuan Xie, Xiao LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title | LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title_full | LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title_fullStr | LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title_full_unstemmed | LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title_short | LncRNA TUSC8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of VEGFA |
title_sort | lncrna tusc8 suppresses the proliferation and migration of esophageal cancer cells by downregulation of vegfa |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489135/ https://www.ncbi.nlm.nih.gov/pubmed/34659529 http://dx.doi.org/10.7150/jca.57814 |
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