Cargando…

RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway

Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression o...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qingyuan, Lai, Qiuhua, He, Chengcheng, Zhang, Haonan, Pan, Xingzhu, Li, Haolin, Yan, Qun, Fang, Yuxin, Liu, Side, Li, Aimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489138/
https://www.ncbi.nlm.nih.gov/pubmed/34659526
http://dx.doi.org/10.7150/jca.51338
_version_ 1784578293207400448
author Li, Qingyuan
Lai, Qiuhua
He, Chengcheng
Zhang, Haonan
Pan, Xingzhu
Li, Haolin
Yan, Qun
Fang, Yuxin
Liu, Side
Li, Aimin
author_facet Li, Qingyuan
Lai, Qiuhua
He, Chengcheng
Zhang, Haonan
Pan, Xingzhu
Li, Haolin
Yan, Qun
Fang, Yuxin
Liu, Side
Li, Aimin
author_sort Li, Qingyuan
collection PubMed
description Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression of RUNX1 in CRC and normal tissues was detected by bioinformatics analysis. Cell proliferation was measured by CCK-8 and clonogenic assays. In vivo tumor progression was assessed with a xenograft mouse model. Cell drug sensitivity tests and flow cytometry were performed to analyze CRC cell chemoresistance. RUNX1, key molecules of the Hedgehog signaling pathway, and ABCG2 were detected by qRT-PCR and Western blotting. Results: RUNX1 expression is upregulated in CRC tissues. RUNX1 enhanced CRC cell resistance to 5-fluorouracil (5-FU), promoted proliferation, and inhibited 5-FU-induced apoptosis. Mechanistically, RUNX1 can activate the Hedgehog signaling pathway and promote the expression of ABCG2 in CRC cells. Conclusions: Our study demonstrated that RUNX1 promotes CRC proliferation and chemoresistance by activating the Hedgehog signaling pathway and ABCG2 expression.
format Online
Article
Text
id pubmed-8489138
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-84891382021-10-15 RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway Li, Qingyuan Lai, Qiuhua He, Chengcheng Zhang, Haonan Pan, Xingzhu Li, Haolin Yan, Qun Fang, Yuxin Liu, Side Li, Aimin J Cancer Research Paper Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression of RUNX1 in CRC and normal tissues was detected by bioinformatics analysis. Cell proliferation was measured by CCK-8 and clonogenic assays. In vivo tumor progression was assessed with a xenograft mouse model. Cell drug sensitivity tests and flow cytometry were performed to analyze CRC cell chemoresistance. RUNX1, key molecules of the Hedgehog signaling pathway, and ABCG2 were detected by qRT-PCR and Western blotting. Results: RUNX1 expression is upregulated in CRC tissues. RUNX1 enhanced CRC cell resistance to 5-fluorouracil (5-FU), promoted proliferation, and inhibited 5-FU-induced apoptosis. Mechanistically, RUNX1 can activate the Hedgehog signaling pathway and promote the expression of ABCG2 in CRC cells. Conclusions: Our study demonstrated that RUNX1 promotes CRC proliferation and chemoresistance by activating the Hedgehog signaling pathway and ABCG2 expression. Ivyspring International Publisher 2021-09-03 /pmc/articles/PMC8489138/ /pubmed/34659526 http://dx.doi.org/10.7150/jca.51338 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Li, Qingyuan
Lai, Qiuhua
He, Chengcheng
Zhang, Haonan
Pan, Xingzhu
Li, Haolin
Yan, Qun
Fang, Yuxin
Liu, Side
Li, Aimin
RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title_full RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title_fullStr RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title_full_unstemmed RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title_short RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
title_sort runx1 regulates the proliferation and chemoresistance of colorectal cancer through the hedgehog signaling pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489138/
https://www.ncbi.nlm.nih.gov/pubmed/34659526
http://dx.doi.org/10.7150/jca.51338
work_keys_str_mv AT liqingyuan runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT laiqiuhua runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT hechengcheng runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT zhanghaonan runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT panxingzhu runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT lihaolin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT yanqun runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT fangyuxin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT liuside runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway
AT liaimin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway