Cargando…
RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway
Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression o...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489138/ https://www.ncbi.nlm.nih.gov/pubmed/34659526 http://dx.doi.org/10.7150/jca.51338 |
_version_ | 1784578293207400448 |
---|---|
author | Li, Qingyuan Lai, Qiuhua He, Chengcheng Zhang, Haonan Pan, Xingzhu Li, Haolin Yan, Qun Fang, Yuxin Liu, Side Li, Aimin |
author_facet | Li, Qingyuan Lai, Qiuhua He, Chengcheng Zhang, Haonan Pan, Xingzhu Li, Haolin Yan, Qun Fang, Yuxin Liu, Side Li, Aimin |
author_sort | Li, Qingyuan |
collection | PubMed |
description | Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression of RUNX1 in CRC and normal tissues was detected by bioinformatics analysis. Cell proliferation was measured by CCK-8 and clonogenic assays. In vivo tumor progression was assessed with a xenograft mouse model. Cell drug sensitivity tests and flow cytometry were performed to analyze CRC cell chemoresistance. RUNX1, key molecules of the Hedgehog signaling pathway, and ABCG2 were detected by qRT-PCR and Western blotting. Results: RUNX1 expression is upregulated in CRC tissues. RUNX1 enhanced CRC cell resistance to 5-fluorouracil (5-FU), promoted proliferation, and inhibited 5-FU-induced apoptosis. Mechanistically, RUNX1 can activate the Hedgehog signaling pathway and promote the expression of ABCG2 in CRC cells. Conclusions: Our study demonstrated that RUNX1 promotes CRC proliferation and chemoresistance by activating the Hedgehog signaling pathway and ABCG2 expression. |
format | Online Article Text |
id | pubmed-8489138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-84891382021-10-15 RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway Li, Qingyuan Lai, Qiuhua He, Chengcheng Zhang, Haonan Pan, Xingzhu Li, Haolin Yan, Qun Fang, Yuxin Liu, Side Li, Aimin J Cancer Research Paper Background: Chemoresistance is one of the main causes of recurrence in colorectal cancer (CRC) patients and leads to a poor prognosis. To characterize RUNX1 expression in colorectal cancer (CRC) and elucidate its mechanistic involvement in the tumor biology of this disease. Methods: The expression of RUNX1 in CRC and normal tissues was detected by bioinformatics analysis. Cell proliferation was measured by CCK-8 and clonogenic assays. In vivo tumor progression was assessed with a xenograft mouse model. Cell drug sensitivity tests and flow cytometry were performed to analyze CRC cell chemoresistance. RUNX1, key molecules of the Hedgehog signaling pathway, and ABCG2 were detected by qRT-PCR and Western blotting. Results: RUNX1 expression is upregulated in CRC tissues. RUNX1 enhanced CRC cell resistance to 5-fluorouracil (5-FU), promoted proliferation, and inhibited 5-FU-induced apoptosis. Mechanistically, RUNX1 can activate the Hedgehog signaling pathway and promote the expression of ABCG2 in CRC cells. Conclusions: Our study demonstrated that RUNX1 promotes CRC proliferation and chemoresistance by activating the Hedgehog signaling pathway and ABCG2 expression. Ivyspring International Publisher 2021-09-03 /pmc/articles/PMC8489138/ /pubmed/34659526 http://dx.doi.org/10.7150/jca.51338 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Qingyuan Lai, Qiuhua He, Chengcheng Zhang, Haonan Pan, Xingzhu Li, Haolin Yan, Qun Fang, Yuxin Liu, Side Li, Aimin RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title | RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title_full | RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title_fullStr | RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title_full_unstemmed | RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title_short | RUNX1 regulates the proliferation and chemoresistance of colorectal cancer through the Hedgehog signaling pathway |
title_sort | runx1 regulates the proliferation and chemoresistance of colorectal cancer through the hedgehog signaling pathway |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489138/ https://www.ncbi.nlm.nih.gov/pubmed/34659526 http://dx.doi.org/10.7150/jca.51338 |
work_keys_str_mv | AT liqingyuan runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT laiqiuhua runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT hechengcheng runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT zhanghaonan runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT panxingzhu runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT lihaolin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT yanqun runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT fangyuxin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT liuside runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway AT liaimin runx1regulatestheproliferationandchemoresistanceofcolorectalcancerthroughthehedgehogsignalingpathway |