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The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active compo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489146/ https://www.ncbi.nlm.nih.gov/pubmed/34659528 http://dx.doi.org/10.7150/jca.54602 |
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author | Han, Guangtao Zhang, Yubiao Liu, Ting Li, Jianping Li, Haohuan |
author_facet | Han, Guangtao Zhang, Yubiao Liu, Ting Li, Jianping Li, Haohuan |
author_sort | Han, Guangtao |
collection | PubMed |
description | Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active components in panax notoginseng and were reported to be capable of inhibiting the growth of several tumors both in vitro and in vivo. However, its effects on osteosarcoma have not been studied yet, which is addressed in this study for the first time. Our results indicated that PNS can inhibit proliferation, invasion and migration of the osteosarcoma cells, while promoting their apoptosis simultaneously. Specifically, PNS caused an increase in mitochondrial membrane potential and the amount of reactive oxygen species. The cell cycle in osteosarcoma cells was arrested in the G0 / G1 phase after PNS treatment. The expression of p53 and other apoptosis-related mitochondrial proteins were promoted. Nevertheless, it was observed that autophagy became less active in osteosarcoma cells when PNS was administered. In a word, PNS were of potential therapeutic significance for osteosarcoma. |
format | Online Article Text |
id | pubmed-8489146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-84891462021-10-15 The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis Han, Guangtao Zhang, Yubiao Liu, Ting Li, Jianping Li, Haohuan J Cancer Research Paper Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active components in panax notoginseng and were reported to be capable of inhibiting the growth of several tumors both in vitro and in vivo. However, its effects on osteosarcoma have not been studied yet, which is addressed in this study for the first time. Our results indicated that PNS can inhibit proliferation, invasion and migration of the osteosarcoma cells, while promoting their apoptosis simultaneously. Specifically, PNS caused an increase in mitochondrial membrane potential and the amount of reactive oxygen species. The cell cycle in osteosarcoma cells was arrested in the G0 / G1 phase after PNS treatment. The expression of p53 and other apoptosis-related mitochondrial proteins were promoted. Nevertheless, it was observed that autophagy became less active in osteosarcoma cells when PNS was administered. In a word, PNS were of potential therapeutic significance for osteosarcoma. Ivyspring International Publisher 2021-08-30 /pmc/articles/PMC8489146/ /pubmed/34659528 http://dx.doi.org/10.7150/jca.54602 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Han, Guangtao Zhang, Yubiao Liu, Ting Li, Jianping Li, Haohuan The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title | The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title_full | The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title_fullStr | The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title_full_unstemmed | The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title_short | The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
title_sort | anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the g(0) / g(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489146/ https://www.ncbi.nlm.nih.gov/pubmed/34659528 http://dx.doi.org/10.7150/jca.54602 |
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