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The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis

Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active compo...

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Autores principales: Han, Guangtao, Zhang, Yubiao, Liu, Ting, Li, Jianping, Li, Haohuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489146/
https://www.ncbi.nlm.nih.gov/pubmed/34659528
http://dx.doi.org/10.7150/jca.54602
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author Han, Guangtao
Zhang, Yubiao
Liu, Ting
Li, Jianping
Li, Haohuan
author_facet Han, Guangtao
Zhang, Yubiao
Liu, Ting
Li, Jianping
Li, Haohuan
author_sort Han, Guangtao
collection PubMed
description Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active components in panax notoginseng and were reported to be capable of inhibiting the growth of several tumors both in vitro and in vivo. However, its effects on osteosarcoma have not been studied yet, which is addressed in this study for the first time. Our results indicated that PNS can inhibit proliferation, invasion and migration of the osteosarcoma cells, while promoting their apoptosis simultaneously. Specifically, PNS caused an increase in mitochondrial membrane potential and the amount of reactive oxygen species. The cell cycle in osteosarcoma cells was arrested in the G0 / G1 phase after PNS treatment. The expression of p53 and other apoptosis-related mitochondrial proteins were promoted. Nevertheless, it was observed that autophagy became less active in osteosarcoma cells when PNS was administered. In a word, PNS were of potential therapeutic significance for osteosarcoma.
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spelling pubmed-84891462021-10-15 The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis Han, Guangtao Zhang, Yubiao Liu, Ting Li, Jianping Li, Haohuan J Cancer Research Paper Osteosarcoma is the most common primary bone malignancy, and current chemotherapy sessions against it often induce severe complications in patients. Thus, it is necessary to develop new and effective antineoplastic agents with fewer side effects. Panax notoginseng saponins (PNS) are the active components in panax notoginseng and were reported to be capable of inhibiting the growth of several tumors both in vitro and in vivo. However, its effects on osteosarcoma have not been studied yet, which is addressed in this study for the first time. Our results indicated that PNS can inhibit proliferation, invasion and migration of the osteosarcoma cells, while promoting their apoptosis simultaneously. Specifically, PNS caused an increase in mitochondrial membrane potential and the amount of reactive oxygen species. The cell cycle in osteosarcoma cells was arrested in the G0 / G1 phase after PNS treatment. The expression of p53 and other apoptosis-related mitochondrial proteins were promoted. Nevertheless, it was observed that autophagy became less active in osteosarcoma cells when PNS was administered. In a word, PNS were of potential therapeutic significance for osteosarcoma. Ivyspring International Publisher 2021-08-30 /pmc/articles/PMC8489146/ /pubmed/34659528 http://dx.doi.org/10.7150/jca.54602 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Han, Guangtao
Zhang, Yubiao
Liu, Ting
Li, Jianping
Li, Haohuan
The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title_full The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title_fullStr The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title_full_unstemmed The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title_short The anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the G(0) / G(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
title_sort anti-osteosarcoma effect from panax notoginseng saponins by inhibiting the g(0) / g(1) phase in the cell cycle and affecting p53-mediated autophagy and mitochondrial apoptosis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489146/
https://www.ncbi.nlm.nih.gov/pubmed/34659528
http://dx.doi.org/10.7150/jca.54602
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