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A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing

Q fever is a zoonotic disease caused by Coxiella burnetii. Most patients have non-specific symptoms at onset. In addition, routine diagnostic tests for C. burnetii are not sensitive, and the bacterium cannot grow in general culture medium. The diagnosis of Q fever therefore poses a challenge. This c...

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Autores principales: Huang, Jinwei, Wang, Rui, Gao, Can, Lü, Yuankai, Cao, Zhuo, Deng, Shanshan, Yue, Changwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489766/
https://www.ncbi.nlm.nih.gov/pubmed/34590876
http://dx.doi.org/10.1177/03000605211025398
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author Huang, Jinwei
Wang, Rui
Gao, Can
Lü, Yuankai
Cao, Zhuo
Deng, Shanshan
Yue, Changwu
author_facet Huang, Jinwei
Wang, Rui
Gao, Can
Lü, Yuankai
Cao, Zhuo
Deng, Shanshan
Yue, Changwu
author_sort Huang, Jinwei
collection PubMed
description Q fever is a zoonotic disease caused by Coxiella burnetii. Most patients have non-specific symptoms at onset. In addition, routine diagnostic tests for C. burnetii are not sensitive, and the bacterium cannot grow in general culture medium. The diagnosis of Q fever therefore poses a challenge. This case study describes a man with a clear history of tick bite who had recurrent fever, pneumonia, and liver damage. Routine tests and bacterial cultures failed to clarify the pathogeny, but laboratory and imaging data suggested infection. After routine tests were exhausted, we detected the presence of C. burnetii in a whole blood sample using next-generation sequencing (NGS). To our knowledge, this is the first report of Q fever associated with Coxiella burnetii detected directly from blood samples in Lishui, China. NGS has revolutionized the diagnosis of infectious diseases, especially those caused by rare or newly discovered pathogens, and patient responses have finally proved its substantial benefits. NGS has important clinical significance for the early diagnosis of chronic Q fever. This proof-of-concept study is worthy of promotion in clinical practice.
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spelling pubmed-84897662021-10-05 A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing Huang, Jinwei Wang, Rui Gao, Can Lü, Yuankai Cao, Zhuo Deng, Shanshan Yue, Changwu J Int Med Res Case Reports Q fever is a zoonotic disease caused by Coxiella burnetii. Most patients have non-specific symptoms at onset. In addition, routine diagnostic tests for C. burnetii are not sensitive, and the bacterium cannot grow in general culture medium. The diagnosis of Q fever therefore poses a challenge. This case study describes a man with a clear history of tick bite who had recurrent fever, pneumonia, and liver damage. Routine tests and bacterial cultures failed to clarify the pathogeny, but laboratory and imaging data suggested infection. After routine tests were exhausted, we detected the presence of C. burnetii in a whole blood sample using next-generation sequencing (NGS). To our knowledge, this is the first report of Q fever associated with Coxiella burnetii detected directly from blood samples in Lishui, China. NGS has revolutionized the diagnosis of infectious diseases, especially those caused by rare or newly discovered pathogens, and patient responses have finally proved its substantial benefits. NGS has important clinical significance for the early diagnosis of chronic Q fever. This proof-of-concept study is worthy of promotion in clinical practice. SAGE Publications 2021-09-30 /pmc/articles/PMC8489766/ /pubmed/34590876 http://dx.doi.org/10.1177/03000605211025398 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Case Reports
Huang, Jinwei
Wang, Rui
Gao, Can
Lü, Yuankai
Cao, Zhuo
Deng, Shanshan
Yue, Changwu
A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title_full A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title_fullStr A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title_full_unstemmed A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title_short A case of tick-transmitted Q fever in Lishui, China diagnosed by next-generation sequencing
title_sort case of tick-transmitted q fever in lishui, china diagnosed by next-generation sequencing
topic Case Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489766/
https://www.ncbi.nlm.nih.gov/pubmed/34590876
http://dx.doi.org/10.1177/03000605211025398
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