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Sperm DNA fragmentation and male age: results of in vitro fertilization treatments
OBJECTIVE: This study aimed to assess the effects of sperm DNA fragmentation in parents belonging to different age groups. The couples included in the study comprised normozoospermic men and infertile women undergoing conventional IVF. METHODS: The results obtained from 163 conventional IVF cycles w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Brazilian Society of Assisted Reproduction
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489813/ https://www.ncbi.nlm.nih.gov/pubmed/34061484 http://dx.doi.org/10.5935/1518-0557.20210018 |
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author | Martínez, Estefanía Bezazián, Constanza Bezazián, Ana Lindl, Karen Peliquero, Anabela Cattaneo, Antonio Gnocchi, Diego Irigoyen, Marcela Tessari, Lautaro Martínez, A. Gustavo |
author_facet | Martínez, Estefanía Bezazián, Constanza Bezazián, Ana Lindl, Karen Peliquero, Anabela Cattaneo, Antonio Gnocchi, Diego Irigoyen, Marcela Tessari, Lautaro Martínez, A. Gustavo |
author_sort | Martínez, Estefanía |
collection | PubMed |
description | OBJECTIVE: This study aimed to assess the effects of sperm DNA fragmentation in parents belonging to different age groups. The couples included in the study comprised normozoospermic men and infertile women undergoing conventional IVF. METHODS: The results obtained from 163 conventional IVF cycles were analyzed retrospectively. The couples enrolled in the study included women aged between 30 and 37 years. Sperm DNA fragmentation was studied using the TUNEL assay. The patients were split into four groups based on male age and sperm DNA fragmentation, as follows: Group 1: ≤39 years and TUNEL assay ≤20%; Group 2: ≤39 years and TUNEL assay >20%; Group 3: ≥40 years and TUNEL assay ≤20%; and Group 4: ≥40 years and TUNEL assay >20%. RESULTS: No significant differences were found in semen parameters or fertilization rates between groups. Groups with <20% sperm DNA fragmentation showed significant differences in other parameters, including higher blastocyst formation rate (Group 1: 63% and Group 3: 60% vs. Group 2: 43% and Group 4: 41%, p<0.05) and higher expanded blastocyst formation rate (Group 1: 42% and Group 3: 40% vs. Group 2: 21% and Group 4: 18%, p<0.05). Miscarriage rate was significantly higher in Group 4 (42% and 46% vs. 5%, 25% and 5% in Groups 1, 2 and 3, respectively, p<0.05). CONCLUSIONS: Our results showed lower blastocyst formation rates from IVF when males had high levels of sperm DNA fragmentation. Higher miscarriage rates were also observed in couples with males aged 40+ years. These results reinforce the need to inform couples with male partners aged 40+ years about the potential risks inherent to fertility treatment. |
format | Online Article Text |
id | pubmed-8489813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Brazilian Society of Assisted Reproduction |
record_format | MEDLINE/PubMed |
spelling | pubmed-84898132021-10-07 Sperm DNA fragmentation and male age: results of in vitro fertilization treatments Martínez, Estefanía Bezazián, Constanza Bezazián, Ana Lindl, Karen Peliquero, Anabela Cattaneo, Antonio Gnocchi, Diego Irigoyen, Marcela Tessari, Lautaro Martínez, A. Gustavo JBRA Assist Reprod Original Article OBJECTIVE: This study aimed to assess the effects of sperm DNA fragmentation in parents belonging to different age groups. The couples included in the study comprised normozoospermic men and infertile women undergoing conventional IVF. METHODS: The results obtained from 163 conventional IVF cycles were analyzed retrospectively. The couples enrolled in the study included women aged between 30 and 37 years. Sperm DNA fragmentation was studied using the TUNEL assay. The patients were split into four groups based on male age and sperm DNA fragmentation, as follows: Group 1: ≤39 years and TUNEL assay ≤20%; Group 2: ≤39 years and TUNEL assay >20%; Group 3: ≥40 years and TUNEL assay ≤20%; and Group 4: ≥40 years and TUNEL assay >20%. RESULTS: No significant differences were found in semen parameters or fertilization rates between groups. Groups with <20% sperm DNA fragmentation showed significant differences in other parameters, including higher blastocyst formation rate (Group 1: 63% and Group 3: 60% vs. Group 2: 43% and Group 4: 41%, p<0.05) and higher expanded blastocyst formation rate (Group 1: 42% and Group 3: 40% vs. Group 2: 21% and Group 4: 18%, p<0.05). Miscarriage rate was significantly higher in Group 4 (42% and 46% vs. 5%, 25% and 5% in Groups 1, 2 and 3, respectively, p<0.05). CONCLUSIONS: Our results showed lower blastocyst formation rates from IVF when males had high levels of sperm DNA fragmentation. Higher miscarriage rates were also observed in couples with males aged 40+ years. These results reinforce the need to inform couples with male partners aged 40+ years about the potential risks inherent to fertility treatment. Brazilian Society of Assisted Reproduction 2021 /pmc/articles/PMC8489813/ /pubmed/34061484 http://dx.doi.org/10.5935/1518-0557.20210018 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Martínez, Estefanía Bezazián, Constanza Bezazián, Ana Lindl, Karen Peliquero, Anabela Cattaneo, Antonio Gnocchi, Diego Irigoyen, Marcela Tessari, Lautaro Martínez, A. Gustavo Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title | Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title_full | Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title_fullStr | Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title_full_unstemmed | Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title_short | Sperm DNA fragmentation and male age: results of in vitro fertilization treatments |
title_sort | sperm dna fragmentation and male age: results of in vitro fertilization treatments |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489813/ https://www.ncbi.nlm.nih.gov/pubmed/34061484 http://dx.doi.org/10.5935/1518-0557.20210018 |
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