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Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection
SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system leading to neurological sequelae. We have developed and characterized two new organotypic cultures from ham...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490365/ https://www.ncbi.nlm.nih.gov/pubmed/34608167 http://dx.doi.org/10.1038/s41467-021-26096-z |
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author | Ferren, Marion Favède, Valérie Decimo, Didier Iampietro, Mathieu Lieberman, Nicole A. P. Weickert, Jean-Luc Pelissier, Rodolphe Mazelier, Magalie Terrier, Olivier Moscona, Anne Porotto, Matteo Greninger, Alexander L. Messaddeq, Nadia Horvat, Branka Mathieu, Cyrille |
author_facet | Ferren, Marion Favède, Valérie Decimo, Didier Iampietro, Mathieu Lieberman, Nicole A. P. Weickert, Jean-Luc Pelissier, Rodolphe Mazelier, Magalie Terrier, Olivier Moscona, Anne Porotto, Matteo Greninger, Alexander L. Messaddeq, Nadia Horvat, Branka Mathieu, Cyrille |
author_sort | Ferren, Marion |
collection | PubMed |
description | SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system leading to neurological sequelae. We have developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer a unique opportunity to study the early steps of viral infection and screening antivirals. These models are not dedicated to investigate how the virus reaches the brain. However, they allow validating the early tropism of the virus in the lungs and demonstrating that SARS-CoV-2 could infect the brainstem and the cerebellum, mainly by targeting granular neurons. Viral infection induces specific interferon and innate immune responses with patterns specific to each organ, along with cell death by apoptosis, necroptosis, and pyroptosis. Overall, our data illustrate the potential of rapid modeling of complex tissue-level interactions during infection by a newly emerged virus. |
format | Online Article Text |
id | pubmed-8490365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84903652021-10-07 Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection Ferren, Marion Favède, Valérie Decimo, Didier Iampietro, Mathieu Lieberman, Nicole A. P. Weickert, Jean-Luc Pelissier, Rodolphe Mazelier, Magalie Terrier, Olivier Moscona, Anne Porotto, Matteo Greninger, Alexander L. Messaddeq, Nadia Horvat, Branka Mathieu, Cyrille Nat Commun Article SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system leading to neurological sequelae. We have developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer a unique opportunity to study the early steps of viral infection and screening antivirals. These models are not dedicated to investigate how the virus reaches the brain. However, they allow validating the early tropism of the virus in the lungs and demonstrating that SARS-CoV-2 could infect the brainstem and the cerebellum, mainly by targeting granular neurons. Viral infection induces specific interferon and innate immune responses with patterns specific to each organ, along with cell death by apoptosis, necroptosis, and pyroptosis. Overall, our data illustrate the potential of rapid modeling of complex tissue-level interactions during infection by a newly emerged virus. Nature Publishing Group UK 2021-10-04 /pmc/articles/PMC8490365/ /pubmed/34608167 http://dx.doi.org/10.1038/s41467-021-26096-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ferren, Marion Favède, Valérie Decimo, Didier Iampietro, Mathieu Lieberman, Nicole A. P. Weickert, Jean-Luc Pelissier, Rodolphe Mazelier, Magalie Terrier, Olivier Moscona, Anne Porotto, Matteo Greninger, Alexander L. Messaddeq, Nadia Horvat, Branka Mathieu, Cyrille Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title | Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title_full | Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title_fullStr | Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title_full_unstemmed | Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title_short | Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection |
title_sort | hamster organotypic modeling of sars-cov-2 lung and brainstem infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490365/ https://www.ncbi.nlm.nih.gov/pubmed/34608167 http://dx.doi.org/10.1038/s41467-021-26096-z |
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