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Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations
This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effects in diab...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490407/ https://www.ncbi.nlm.nih.gov/pubmed/34608232 http://dx.doi.org/10.1038/s41598-021-99136-9 |
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author | Porras Hernández, Ana María Barbe, Laurent Pohlit, Hannah Tenje, Maria Antfolk, Maria |
author_facet | Porras Hernández, Ana María Barbe, Laurent Pohlit, Hannah Tenje, Maria Antfolk, Maria |
author_sort | Porras Hernández, Ana María |
collection | PubMed |
description | This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effects in diabetes. We prepared hydrogels of hyaluronic acid a natural biomaterial that does not naturally support endothelial cell adhesion, and specifically functionalised RGD peptides into lines using UV-mediated linkage. The width of the lines was varied from 10 to 100 µm. We evaluated cell alignment by measuring the nuclei, cell, and F-actin orientations, and the nuclei and cell eccentricity via immunofluorescent staining and image analysis. We found that the brain microvascular endothelial cells aligned and elongated to these physical constraints for all line widths. In addition, we also observed that varying the cell medium glucose levels affected the cell alignment along the patterns. We believe our results may provide a platform for further studies on the impact of altered glucose levels in cardiovascular disease. |
format | Online Article Text |
id | pubmed-8490407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84904072021-10-05 Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations Porras Hernández, Ana María Barbe, Laurent Pohlit, Hannah Tenje, Maria Antfolk, Maria Sci Rep Article This work reports on an effort to decipher the alignment of brain microvasculature endothelial cells to physical constrains generated via adhesion control on hydrogel surfaces and explore the corresponding responses upon glucose level variations emulating the hypo- and hyperglycaemic effects in diabetes. We prepared hydrogels of hyaluronic acid a natural biomaterial that does not naturally support endothelial cell adhesion, and specifically functionalised RGD peptides into lines using UV-mediated linkage. The width of the lines was varied from 10 to 100 µm. We evaluated cell alignment by measuring the nuclei, cell, and F-actin orientations, and the nuclei and cell eccentricity via immunofluorescent staining and image analysis. We found that the brain microvascular endothelial cells aligned and elongated to these physical constraints for all line widths. In addition, we also observed that varying the cell medium glucose levels affected the cell alignment along the patterns. We believe our results may provide a platform for further studies on the impact of altered glucose levels in cardiovascular disease. Nature Publishing Group UK 2021-10-04 /pmc/articles/PMC8490407/ /pubmed/34608232 http://dx.doi.org/10.1038/s41598-021-99136-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Porras Hernández, Ana María Barbe, Laurent Pohlit, Hannah Tenje, Maria Antfolk, Maria Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title | Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title_full | Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title_fullStr | Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title_full_unstemmed | Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title_short | Brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
title_sort | brain microvasculature endothelial cell orientation on micropatterned hydrogels is affected by glucose level variations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490407/ https://www.ncbi.nlm.nih.gov/pubmed/34608232 http://dx.doi.org/10.1038/s41598-021-99136-9 |
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