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Programmable nano-reactors for stochastic sensing

Chemical reactions of single molecules, caused by rapid formation or breaking of chemical bonds, are difficult to observe even with state-of-the-art instruments. A biological nanopore can be engineered into a single molecule reactor, capable of detecting the binding of a monatomic ion or the transie...

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Autores principales: Jia, Wendong, Hu, Chengzhen, Wang, Yuqin, Gu, Yuming, Qian, Guangrui, Du, Xiaoyu, Wang, Liying, Liu, Yao, Cao, Jiao, Zhang, Shanyu, Yan, Shuanghong, Zhang, Panke, Ma, Jing, Chen, Hong-Yuan, Huang, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490433/
https://www.ncbi.nlm.nih.gov/pubmed/34608151
http://dx.doi.org/10.1038/s41467-021-26054-9
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author Jia, Wendong
Hu, Chengzhen
Wang, Yuqin
Gu, Yuming
Qian, Guangrui
Du, Xiaoyu
Wang, Liying
Liu, Yao
Cao, Jiao
Zhang, Shanyu
Yan, Shuanghong
Zhang, Panke
Ma, Jing
Chen, Hong-Yuan
Huang, Shuo
author_facet Jia, Wendong
Hu, Chengzhen
Wang, Yuqin
Gu, Yuming
Qian, Guangrui
Du, Xiaoyu
Wang, Liying
Liu, Yao
Cao, Jiao
Zhang, Shanyu
Yan, Shuanghong
Zhang, Panke
Ma, Jing
Chen, Hong-Yuan
Huang, Shuo
author_sort Jia, Wendong
collection PubMed
description Chemical reactions of single molecules, caused by rapid formation or breaking of chemical bonds, are difficult to observe even with state-of-the-art instruments. A biological nanopore can be engineered into a single molecule reactor, capable of detecting the binding of a monatomic ion or the transient appearance of chemical intermediates. Pore engineering of this type is however technically challenging, which has significantly restricted further development of this technique. We propose a versatile strategy, “programmable nano-reactors for stochastic sensing” (PNRSS), by which a variety of single molecule reactions of hydrogen peroxide, metal ions, ethylene glycol, glycerol, lactic acid, vitamins, catecholamines or nucleoside analogues can be observed directly. PNRSS presents a refined sensing resolution which can be further enhanced by an artificial intelligence algorithm. Remdesivir, a nucleoside analogue and an investigational anti-viral drug used to treat COVID-19, can be distinguished from its active triphosphate form by PNRSS, suggesting applications in pharmacokinetics or drug screening.
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spelling pubmed-84904332021-10-07 Programmable nano-reactors for stochastic sensing Jia, Wendong Hu, Chengzhen Wang, Yuqin Gu, Yuming Qian, Guangrui Du, Xiaoyu Wang, Liying Liu, Yao Cao, Jiao Zhang, Shanyu Yan, Shuanghong Zhang, Panke Ma, Jing Chen, Hong-Yuan Huang, Shuo Nat Commun Article Chemical reactions of single molecules, caused by rapid formation or breaking of chemical bonds, are difficult to observe even with state-of-the-art instruments. A biological nanopore can be engineered into a single molecule reactor, capable of detecting the binding of a monatomic ion or the transient appearance of chemical intermediates. Pore engineering of this type is however technically challenging, which has significantly restricted further development of this technique. We propose a versatile strategy, “programmable nano-reactors for stochastic sensing” (PNRSS), by which a variety of single molecule reactions of hydrogen peroxide, metal ions, ethylene glycol, glycerol, lactic acid, vitamins, catecholamines or nucleoside analogues can be observed directly. PNRSS presents a refined sensing resolution which can be further enhanced by an artificial intelligence algorithm. Remdesivir, a nucleoside analogue and an investigational anti-viral drug used to treat COVID-19, can be distinguished from its active triphosphate form by PNRSS, suggesting applications in pharmacokinetics or drug screening. Nature Publishing Group UK 2021-10-04 /pmc/articles/PMC8490433/ /pubmed/34608151 http://dx.doi.org/10.1038/s41467-021-26054-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jia, Wendong
Hu, Chengzhen
Wang, Yuqin
Gu, Yuming
Qian, Guangrui
Du, Xiaoyu
Wang, Liying
Liu, Yao
Cao, Jiao
Zhang, Shanyu
Yan, Shuanghong
Zhang, Panke
Ma, Jing
Chen, Hong-Yuan
Huang, Shuo
Programmable nano-reactors for stochastic sensing
title Programmable nano-reactors for stochastic sensing
title_full Programmable nano-reactors for stochastic sensing
title_fullStr Programmable nano-reactors for stochastic sensing
title_full_unstemmed Programmable nano-reactors for stochastic sensing
title_short Programmable nano-reactors for stochastic sensing
title_sort programmable nano-reactors for stochastic sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490433/
https://www.ncbi.nlm.nih.gov/pubmed/34608151
http://dx.doi.org/10.1038/s41467-021-26054-9
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