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Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation

Pulsed-field ablation (PFA) had potential advantages in atrial fibrillation ablation, and we aim to confirm the optimal parameter and target of PFA for atrial fibrillation. Two ablation modes in vitro of single-cell system (ablation in electrode cup) and monolayer cell system (ablation in inserts wi...

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Autores principales: Ye, Xuying, Liu, Shangzhong, Yin, Huijuan, He, Qiang, Xue, Zhixiao, Lu, Chengzhi, Su, Siying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490619/
https://www.ncbi.nlm.nih.gov/pubmed/34621795
http://dx.doi.org/10.3389/fcvm.2021.690092
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author Ye, Xuying
Liu, Shangzhong
Yin, Huijuan
He, Qiang
Xue, Zhixiao
Lu, Chengzhi
Su, Siying
author_facet Ye, Xuying
Liu, Shangzhong
Yin, Huijuan
He, Qiang
Xue, Zhixiao
Lu, Chengzhi
Su, Siying
author_sort Ye, Xuying
collection PubMed
description Pulsed-field ablation (PFA) had potential advantages in atrial fibrillation ablation, and we aim to confirm the optimal parameter and target of PFA for atrial fibrillation. Two ablation modes in vitro of single-cell system (ablation in electrode cup) and monolayer cell system (ablation in inserts with electrode tips) were established to perform PFA for myocardial cell H9C2 and smooth muscle cell A7r5. Ablation effect, calcium ion influx, the expression of Cx45, and surface morphological change were observed. Three Bama minipigs were used to verify the in vivo ablation effect of PFA. In monolayer cell system, H9C2 was significantly sensitive to PFA compared with A7r5, with shrinking of the whole monolayer. The ablation effect of bidirectional pulse was weaker than that of the two mono-polar pulses. Expressed Cx45 proteins were increased in H9C2 but decreased in A7r5 cells. Bidirectional PFA performed on Bama minipigs was able to effectively block electrical activity from the pulmonary vein to the atrium with week muscle contraction, not generating pulmonary vein stenosis. Bidirectional PFA was able to significantly ablate myocardial cells, maintain cell–cell connection, and reduce muscle contraction, which was a kind of optimized PFA strategy for atrial fibrillation.
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spelling pubmed-84906192021-10-06 Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation Ye, Xuying Liu, Shangzhong Yin, Huijuan He, Qiang Xue, Zhixiao Lu, Chengzhi Su, Siying Front Cardiovasc Med Cardiovascular Medicine Pulsed-field ablation (PFA) had potential advantages in atrial fibrillation ablation, and we aim to confirm the optimal parameter and target of PFA for atrial fibrillation. Two ablation modes in vitro of single-cell system (ablation in electrode cup) and monolayer cell system (ablation in inserts with electrode tips) were established to perform PFA for myocardial cell H9C2 and smooth muscle cell A7r5. Ablation effect, calcium ion influx, the expression of Cx45, and surface morphological change were observed. Three Bama minipigs were used to verify the in vivo ablation effect of PFA. In monolayer cell system, H9C2 was significantly sensitive to PFA compared with A7r5, with shrinking of the whole monolayer. The ablation effect of bidirectional pulse was weaker than that of the two mono-polar pulses. Expressed Cx45 proteins were increased in H9C2 but decreased in A7r5 cells. Bidirectional PFA performed on Bama minipigs was able to effectively block electrical activity from the pulmonary vein to the atrium with week muscle contraction, not generating pulmonary vein stenosis. Bidirectional PFA was able to significantly ablate myocardial cells, maintain cell–cell connection, and reduce muscle contraction, which was a kind of optimized PFA strategy for atrial fibrillation. Frontiers Media S.A. 2021-09-21 /pmc/articles/PMC8490619/ /pubmed/34621795 http://dx.doi.org/10.3389/fcvm.2021.690092 Text en Copyright © 2021 Ye, Liu, Yin, He, Xue, Lu and Su. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Ye, Xuying
Liu, Shangzhong
Yin, Huijuan
He, Qiang
Xue, Zhixiao
Lu, Chengzhi
Su, Siying
Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title_full Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title_fullStr Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title_full_unstemmed Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title_short Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation
title_sort study on optimal parameter and target for pulsed-field ablation of atrial fibrillation
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490619/
https://www.ncbi.nlm.nih.gov/pubmed/34621795
http://dx.doi.org/10.3389/fcvm.2021.690092
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