Cargando…

Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status

BACKGROUND: Streptococcus species are predominant members of the oral microbiota in both health and diseased conditions. The purpose of the present study was to explore if different ecological characteristics, such as oxygen availability and presence of periodontitis, associates with transcriptional...

Descripción completa

Detalles Bibliográficos
Autores principales: Belstrøm, Daniel, Constancias, Florentin, Markvart, Merete, Sikora, Martin, Sørensen, Christiane Elisabeth, Givskov, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490622/
https://www.ncbi.nlm.nih.gov/pubmed/34621696
http://dx.doi.org/10.3389/fcimb.2021.752664
_version_ 1784578552080891904
author Belstrøm, Daniel
Constancias, Florentin
Markvart, Merete
Sikora, Martin
Sørensen, Christiane Elisabeth
Givskov, Michael
author_facet Belstrøm, Daniel
Constancias, Florentin
Markvart, Merete
Sikora, Martin
Sørensen, Christiane Elisabeth
Givskov, Michael
author_sort Belstrøm, Daniel
collection PubMed
description BACKGROUND: Streptococcus species are predominant members of the oral microbiota in both health and diseased conditions. The purpose of the present study was to explore if different ecological characteristics, such as oxygen availability and presence of periodontitis, associates with transcriptional activity of predominant members of genus Streptococcus. We tested the hypothesis that genetically closely related Streptococcus species express different transcriptional activities in samples collected from environments with critically different ecological conditions determined by site and inflammatory status. METHODS: Metagenomic and metatranscriptomic data was retrieved from 66 oral samples, subgingival plaque (n=22), tongue scrapings (n=22) and stimulated saliva (n=22) collected from patients with periodontitis (n=11) and orally healthy individuals (n=11). Species-specific transcriptional activity was computed as Log2(RNA/DNA), and transcriptional activity of predominant Streptococcus species was compared between multiple samples collected from different sites in the same individual, and between individuals with different oral health status. RESULTS: The predominant Streptococcus species were identified with a site-specific colonization pattern of the tongue and the subgingival plaque. A total of 11, 4 and 2 pathways expressed by S. parasanguinis, S. infantis and S. salivarius, respectively, were recorded with significantly higher transcriptional activity in saliva than in tongue biofilm in healthy individuals. In addition, 18 pathways, including pathways involved in synthesis of peptidoglycan, amino acid biosynthesis, glycolysis and purine nucleotide biosynthesis expressed by S. parasanguinis and 3 pathways expressed by S. salivarius were identified with significantly less transcriptional activity in patients with periodontitis. CONCLUSION: Data from the present study significantly demonstrates the association of site-specific ecological conditions and presence of periodontitis with transcriptional activity of the predominant Streptococcus species of the oral microbiota. In particular, pathways expressed by S. parasanguinis being involved in peptidoglycan, amino acid biosynthesis, glycolysis, and purine nucleotide biosynthesis were identified to be significantly associated with oral site and/or inflammation status.
format Online
Article
Text
id pubmed-8490622
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84906222021-10-06 Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status Belstrøm, Daniel Constancias, Florentin Markvart, Merete Sikora, Martin Sørensen, Christiane Elisabeth Givskov, Michael Front Cell Infect Microbiol Cellular and Infection Microbiology BACKGROUND: Streptococcus species are predominant members of the oral microbiota in both health and diseased conditions. The purpose of the present study was to explore if different ecological characteristics, such as oxygen availability and presence of periodontitis, associates with transcriptional activity of predominant members of genus Streptococcus. We tested the hypothesis that genetically closely related Streptococcus species express different transcriptional activities in samples collected from environments with critically different ecological conditions determined by site and inflammatory status. METHODS: Metagenomic and metatranscriptomic data was retrieved from 66 oral samples, subgingival plaque (n=22), tongue scrapings (n=22) and stimulated saliva (n=22) collected from patients with periodontitis (n=11) and orally healthy individuals (n=11). Species-specific transcriptional activity was computed as Log2(RNA/DNA), and transcriptional activity of predominant Streptococcus species was compared between multiple samples collected from different sites in the same individual, and between individuals with different oral health status. RESULTS: The predominant Streptococcus species were identified with a site-specific colonization pattern of the tongue and the subgingival plaque. A total of 11, 4 and 2 pathways expressed by S. parasanguinis, S. infantis and S. salivarius, respectively, were recorded with significantly higher transcriptional activity in saliva than in tongue biofilm in healthy individuals. In addition, 18 pathways, including pathways involved in synthesis of peptidoglycan, amino acid biosynthesis, glycolysis and purine nucleotide biosynthesis expressed by S. parasanguinis and 3 pathways expressed by S. salivarius were identified with significantly less transcriptional activity in patients with periodontitis. CONCLUSION: Data from the present study significantly demonstrates the association of site-specific ecological conditions and presence of periodontitis with transcriptional activity of the predominant Streptococcus species of the oral microbiota. In particular, pathways expressed by S. parasanguinis being involved in peptidoglycan, amino acid biosynthesis, glycolysis, and purine nucleotide biosynthesis were identified to be significantly associated with oral site and/or inflammation status. Frontiers Media S.A. 2021-09-21 /pmc/articles/PMC8490622/ /pubmed/34621696 http://dx.doi.org/10.3389/fcimb.2021.752664 Text en Copyright © 2021 Belstrøm, Constancias, Markvart, Sikora, Sørensen and Givskov https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Belstrøm, Daniel
Constancias, Florentin
Markvart, Merete
Sikora, Martin
Sørensen, Christiane Elisabeth
Givskov, Michael
Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title_full Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title_fullStr Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title_full_unstemmed Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title_short Transcriptional Activity of Predominant Streptococcus Species at Multiple Oral Sites Associate With Periodontal Status
title_sort transcriptional activity of predominant streptococcus species at multiple oral sites associate with periodontal status
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490622/
https://www.ncbi.nlm.nih.gov/pubmed/34621696
http://dx.doi.org/10.3389/fcimb.2021.752664
work_keys_str_mv AT belstrømdaniel transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus
AT constanciasflorentin transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus
AT markvartmerete transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus
AT sikoramartin transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus
AT sørensenchristianeelisabeth transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus
AT givskovmichael transcriptionalactivityofpredominantstreptococcusspeciesatmultipleoralsitesassociatewithperiodontalstatus