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Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies

Fecal microbiota transplantation (FMT) has been widely recognized as an approach to determine the microbiome’s causal role in gut dysbiosis-related disease models and as a novel disease-modifying therapy. Despite potential beneficial FMT results in various disease models, there is a variation and co...

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Autores principales: Bokoliya, Suresh C., Dorsett, Yair, Panier, Hunter, Zhou, Yanjiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490673/
https://www.ncbi.nlm.nih.gov/pubmed/34621688
http://dx.doi.org/10.3389/fcimb.2021.711055
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author Bokoliya, Suresh C.
Dorsett, Yair
Panier, Hunter
Zhou, Yanjiao
author_facet Bokoliya, Suresh C.
Dorsett, Yair
Panier, Hunter
Zhou, Yanjiao
author_sort Bokoliya, Suresh C.
collection PubMed
description Fecal microbiota transplantation (FMT) has been widely recognized as an approach to determine the microbiome’s causal role in gut dysbiosis-related disease models and as a novel disease-modifying therapy. Despite potential beneficial FMT results in various disease models, there is a variation and complexity in procedural agreement among research groups for performing FMT. The viability of the microbiome in feces and its successful transfer depends on various aspects of donors, recipients, and lab settings. This review focuses on the technical practices of FMT in animal studies. We first document crucial factors required for collecting, handling, and processing donor fecal microbiota for FMT. Then, we detail the description of gut microbiota depletion methods, FMT dosages, and routes of FMT administrations in recipients. In the end, we describe assessments of success rates of FMT with sustainability. It is critical to work under the anaerobic condition to preserve as much of the viability of bacteria. Utilization of germ- free mice or depletion of recipient gut microbiota by antibiotics or polyethylene glycol are two common recipient preparation approaches to achieve better engraftment. Oral-gastric gavage preferred by most researchers for fast and effective administration of FMT in mice. Overall, this review highlights various methods that may lead to developing the standard and reproducible protocol for FMT.
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spelling pubmed-84906732021-10-06 Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies Bokoliya, Suresh C. Dorsett, Yair Panier, Hunter Zhou, Yanjiao Front Cell Infect Microbiol Cellular and Infection Microbiology Fecal microbiota transplantation (FMT) has been widely recognized as an approach to determine the microbiome’s causal role in gut dysbiosis-related disease models and as a novel disease-modifying therapy. Despite potential beneficial FMT results in various disease models, there is a variation and complexity in procedural agreement among research groups for performing FMT. The viability of the microbiome in feces and its successful transfer depends on various aspects of donors, recipients, and lab settings. This review focuses on the technical practices of FMT in animal studies. We first document crucial factors required for collecting, handling, and processing donor fecal microbiota for FMT. Then, we detail the description of gut microbiota depletion methods, FMT dosages, and routes of FMT administrations in recipients. In the end, we describe assessments of success rates of FMT with sustainability. It is critical to work under the anaerobic condition to preserve as much of the viability of bacteria. Utilization of germ- free mice or depletion of recipient gut microbiota by antibiotics or polyethylene glycol are two common recipient preparation approaches to achieve better engraftment. Oral-gastric gavage preferred by most researchers for fast and effective administration of FMT in mice. Overall, this review highlights various methods that may lead to developing the standard and reproducible protocol for FMT. Frontiers Media S.A. 2021-09-21 /pmc/articles/PMC8490673/ /pubmed/34621688 http://dx.doi.org/10.3389/fcimb.2021.711055 Text en Copyright © 2021 Bokoliya, Dorsett, Panier and Zhou https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Bokoliya, Suresh C.
Dorsett, Yair
Panier, Hunter
Zhou, Yanjiao
Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title_full Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title_fullStr Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title_full_unstemmed Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title_short Procedures for Fecal Microbiota Transplantation in Murine Microbiome Studies
title_sort procedures for fecal microbiota transplantation in murine microbiome studies
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490673/
https://www.ncbi.nlm.nih.gov/pubmed/34621688
http://dx.doi.org/10.3389/fcimb.2021.711055
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