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Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging

The localization and measurement of neuronal activity magnitude at high spatial and temporal resolution are essential for mapping and better understanding neuronal systems and mechanisms. One such example is the generation of retinotopic maps, which correlates localized retinal stimulation with the...

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Autores principales: Carmi, Ori, Gross, Adi, Ivzan, Nadav, Franca, Lamberto La, Farah, Nairouz, Zalevsky, Zeev, Mandel, Yossi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490879/
https://www.ncbi.nlm.nih.gov/pubmed/34621157
http://dx.doi.org/10.3389/fncel.2021.713538
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author Carmi, Ori
Gross, Adi
Ivzan, Nadav
Franca, Lamberto La
Farah, Nairouz
Zalevsky, Zeev
Mandel, Yossi
author_facet Carmi, Ori
Gross, Adi
Ivzan, Nadav
Franca, Lamberto La
Farah, Nairouz
Zalevsky, Zeev
Mandel, Yossi
author_sort Carmi, Ori
collection PubMed
description The localization and measurement of neuronal activity magnitude at high spatial and temporal resolution are essential for mapping and better understanding neuronal systems and mechanisms. One such example is the generation of retinotopic maps, which correlates localized retinal stimulation with the corresponding specific visual cortex responses. Here we evaluated and compared seven different methods for extracting and localizing cortical responses from voltage-sensitive dye imaging recordings, elicited by visual stimuli projected directly on the rat retina by a customized projection system. The performance of these methods was evaluated both qualitatively and quantitatively by means of two cluster separation metrics, namely, the (adjusted) Silhouette Index (SI) and the (adjusted) Davies-Bouldin Index (DBI). These metrics were validated using simulated data, which showed that Temporally Structured Component Analysis (TSCA) outperformed all other analysis methods for localizing cortical responses and generating high-resolution retinotopic maps. The analysis methods, as well as the use of cluster separation metrics proposed here, can facilitate future research aiming to localize specific activity at high resolution in the visual cortex or other brain areas.
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spelling pubmed-84908792021-10-06 Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging Carmi, Ori Gross, Adi Ivzan, Nadav Franca, Lamberto La Farah, Nairouz Zalevsky, Zeev Mandel, Yossi Front Cell Neurosci Neuroscience The localization and measurement of neuronal activity magnitude at high spatial and temporal resolution are essential for mapping and better understanding neuronal systems and mechanisms. One such example is the generation of retinotopic maps, which correlates localized retinal stimulation with the corresponding specific visual cortex responses. Here we evaluated and compared seven different methods for extracting and localizing cortical responses from voltage-sensitive dye imaging recordings, elicited by visual stimuli projected directly on the rat retina by a customized projection system. The performance of these methods was evaluated both qualitatively and quantitatively by means of two cluster separation metrics, namely, the (adjusted) Silhouette Index (SI) and the (adjusted) Davies-Bouldin Index (DBI). These metrics were validated using simulated data, which showed that Temporally Structured Component Analysis (TSCA) outperformed all other analysis methods for localizing cortical responses and generating high-resolution retinotopic maps. The analysis methods, as well as the use of cluster separation metrics proposed here, can facilitate future research aiming to localize specific activity at high resolution in the visual cortex or other brain areas. Frontiers Media S.A. 2021-09-21 /pmc/articles/PMC8490879/ /pubmed/34621157 http://dx.doi.org/10.3389/fncel.2021.713538 Text en Copyright © 2021 Carmi, Gross, Ivzan, Franca, Farah, Zalevsky and Mandel. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Carmi, Ori
Gross, Adi
Ivzan, Nadav
Franca, Lamberto La
Farah, Nairouz
Zalevsky, Zeev
Mandel, Yossi
Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title_full Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title_fullStr Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title_full_unstemmed Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title_short Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging
title_sort evaluation and optimization of methods for generating high-resolution retinotopic maps using visual cortex voltage-sensitive dye imaging
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490879/
https://www.ncbi.nlm.nih.gov/pubmed/34621157
http://dx.doi.org/10.3389/fncel.2021.713538
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