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Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels
Acute lung injury (ALI) is featured with severe lung edema at the early exudative phase, resulting from the imbalance of alveolar fluid turnover and clearance. Mesenchymal stem cells (MSCs) belong to multipotent stem cells, which have shown potential therapeutic effects during ALI. Of note, MSC‐cond...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491198/ https://www.ncbi.nlm.nih.gov/pubmed/34766129 http://dx.doi.org/10.1002/mco2.40 |
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author | Ding, Yan Cui, Yong Hou, Yapeng Nie, Hongguang |
author_facet | Ding, Yan Cui, Yong Hou, Yapeng Nie, Hongguang |
author_sort | Ding, Yan |
collection | PubMed |
description | Acute lung injury (ALI) is featured with severe lung edema at the early exudative phase, resulting from the imbalance of alveolar fluid turnover and clearance. Mesenchymal stem cells (MSCs) belong to multipotent stem cells, which have shown potential therapeutic effects during ALI. Of note, MSC‐conditioned medium (MSC‐CM) improved alveolar fluid clearance (AFC) in vivo, whereas the involvement of miRNAs is seldom known. We thus aim to explore the roles of miR‐214 in facilitating MSC‐CM mediated fluid resolution of impaired AFC. In this study, AFC was increased significantly by intratracheally administrated MSC‐CM in lipopolysaccharide‐treated mice. MSC‐CM augmented amiloride‐sensitive currents in intact H441 monolayers, and increased α‐epithelial sodium channel (α‐ENaC) expression level in H441 and mouse alveolar type 2 epithelial cells. Meanwhile, MSC‐CM increased the expression of miR‐214, which may participate in regulating ENaC expression and function. Our results suggested that MSC‐CM enhanced AFC in ALI mice in vivo through a novel mechanism, involving miR‐214 regulation of ENaC. |
format | Online Article Text |
id | pubmed-8491198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84911982021-11-10 Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels Ding, Yan Cui, Yong Hou, Yapeng Nie, Hongguang MedComm (2020) Original Articles Acute lung injury (ALI) is featured with severe lung edema at the early exudative phase, resulting from the imbalance of alveolar fluid turnover and clearance. Mesenchymal stem cells (MSCs) belong to multipotent stem cells, which have shown potential therapeutic effects during ALI. Of note, MSC‐conditioned medium (MSC‐CM) improved alveolar fluid clearance (AFC) in vivo, whereas the involvement of miRNAs is seldom known. We thus aim to explore the roles of miR‐214 in facilitating MSC‐CM mediated fluid resolution of impaired AFC. In this study, AFC was increased significantly by intratracheally administrated MSC‐CM in lipopolysaccharide‐treated mice. MSC‐CM augmented amiloride‐sensitive currents in intact H441 monolayers, and increased α‐epithelial sodium channel (α‐ENaC) expression level in H441 and mouse alveolar type 2 epithelial cells. Meanwhile, MSC‐CM increased the expression of miR‐214, which may participate in regulating ENaC expression and function. Our results suggested that MSC‐CM enhanced AFC in ALI mice in vivo through a novel mechanism, involving miR‐214 regulation of ENaC. John Wiley and Sons Inc. 2020-11-12 /pmc/articles/PMC8491198/ /pubmed/34766129 http://dx.doi.org/10.1002/mco2.40 Text en © 2020 The Authors. MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ding, Yan Cui, Yong Hou, Yapeng Nie, Hongguang Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title | Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title_full | Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title_fullStr | Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title_full_unstemmed | Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title_short | Bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via miR‐214‐activating epithelial sodium channels |
title_sort | bone marrow mesenchymal stem cell‐conditioned medium facilitates fluid resolution via mir‐214‐activating epithelial sodium channels |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491198/ https://www.ncbi.nlm.nih.gov/pubmed/34766129 http://dx.doi.org/10.1002/mco2.40 |
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