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Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study

AIMS: The aim of the current study was to examine the effects of diode laser (970 nm) when used as adjunct to mechanical debridement on reattachment of fibroblast cell cultures to the root surface. MATERIALS AND METHODS: Eighteen extracted single-rooted human teeth were used in the study. Briefly, t...

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Autores principales: Savita, A M, Nageswaran, G R, Reddy, Roopa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491338/
https://www.ncbi.nlm.nih.gov/pubmed/34703124
http://dx.doi.org/10.4103/0973-029X.325129
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author Savita, A M
Nageswaran, G R
Reddy, Roopa
author_facet Savita, A M
Nageswaran, G R
Reddy, Roopa
author_sort Savita, A M
collection PubMed
description AIMS: The aim of the current study was to examine the effects of diode laser (970 nm) when used as adjunct to mechanical debridement on reattachment of fibroblast cell cultures to the root surface. MATERIALS AND METHODS: Eighteen extracted single-rooted human teeth were used in the study. Briefly, the anatomical crowns were removed; roots were sectioned and preserved in antiseptic solution. The sections were divided into three groups: (i) scaling and root planing (SRP), (ii) SRP followed by diode laser exposure and (iii) untreated control group. All the sections were inoculated with NIH 3T3 mouse fibroblast cell lines and cell attachment was confirmed by scanning electron microscopy analysis and growth was quantitatively assessed by MTT assay after days 1, 3 and 5. RESULTS: On intragroup analysis, there was significant cell proliferation seen on day 1 and day 5. However, no significant differences were observed between the treatment groups at the various time points. CONCLUSION: 970-nm diode laser exposure had a positive effect in enhancing the fibroblast reattachment to root surfaces. However, to ascertain the role of laser treatment in enhancing the rapid cell growth compared to SRP, further quantitative studies with more sample numbers are required.
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spelling pubmed-84913382021-10-25 Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study Savita, A M Nageswaran, G R Reddy, Roopa J Oral Maxillofac Pathol Original Article AIMS: The aim of the current study was to examine the effects of diode laser (970 nm) when used as adjunct to mechanical debridement on reattachment of fibroblast cell cultures to the root surface. MATERIALS AND METHODS: Eighteen extracted single-rooted human teeth were used in the study. Briefly, the anatomical crowns were removed; roots were sectioned and preserved in antiseptic solution. The sections were divided into three groups: (i) scaling and root planing (SRP), (ii) SRP followed by diode laser exposure and (iii) untreated control group. All the sections were inoculated with NIH 3T3 mouse fibroblast cell lines and cell attachment was confirmed by scanning electron microscopy analysis and growth was quantitatively assessed by MTT assay after days 1, 3 and 5. RESULTS: On intragroup analysis, there was significant cell proliferation seen on day 1 and day 5. However, no significant differences were observed between the treatment groups at the various time points. CONCLUSION: 970-nm diode laser exposure had a positive effect in enhancing the fibroblast reattachment to root surfaces. However, to ascertain the role of laser treatment in enhancing the rapid cell growth compared to SRP, further quantitative studies with more sample numbers are required. Medknow Publications & Media Pvt Ltd 2021 2021-08-31 /pmc/articles/PMC8491338/ /pubmed/34703124 http://dx.doi.org/10.4103/0973-029X.325129 Text en Copyright: © 2021 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Savita, A M
Nageswaran, G R
Reddy, Roopa
Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title_full Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title_fullStr Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title_full_unstemmed Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title_short Comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: An in vitro study
title_sort comparative analysis of cytocompatible properties of the root surface exposed to diode laser (970 nm) as adjunct to scaling and root planing: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491338/
https://www.ncbi.nlm.nih.gov/pubmed/34703124
http://dx.doi.org/10.4103/0973-029X.325129
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