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Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study

Dengue has become endemic in Pakistan with annual recurrence. A sudden increase in the dengue cases was reported from Rawalpindi in 2016, while an outbreak occurred for the first time in Peshawar in 2017. Therefore, a multi-center study was carried out to determine the circulating dengue virus (DENV...

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Autores principales: Raza, Faiz Ahmed, Javed, Hasnain, Khan, Muhammad Mujeeb, Ullah, Obaid, Fatima, Areeba, Zaheer, Muhammad, Mohsin, Saima, Hasnain, Shahida, Khalid, Ruqyya, Salam, Arslan Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491885/
https://www.ncbi.nlm.nih.gov/pubmed/34555034
http://dx.doi.org/10.1371/journal.pntd.0009802
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author Raza, Faiz Ahmed
Javed, Hasnain
Khan, Muhammad Mujeeb
Ullah, Obaid
Fatima, Areeba
Zaheer, Muhammad
Mohsin, Saima
Hasnain, Shahida
Khalid, Ruqyya
Salam, Arslan Ahmed
author_facet Raza, Faiz Ahmed
Javed, Hasnain
Khan, Muhammad Mujeeb
Ullah, Obaid
Fatima, Areeba
Zaheer, Muhammad
Mohsin, Saima
Hasnain, Shahida
Khalid, Ruqyya
Salam, Arslan Ahmed
author_sort Raza, Faiz Ahmed
collection PubMed
description Dengue has become endemic in Pakistan with annual recurrence. A sudden increase in the dengue cases was reported from Rawalpindi in 2016, while an outbreak occurred for the first time in Peshawar in 2017. Therefore, a multi-center study was carried out to determine the circulating dengue virus (DENV) serotypes and Chikungunya virus (CHIKV) co-infection in Lahore, Rawalpindi, and Peshawar cities in 2016–18. A hospital-based cross-sectional study was carried out in Lahore and Rawalpindi in 2016–18, while a community-based study was carried out in Peshawar in 2017. The study participants were tested for dengue NS1 antigen using an immunochromatographic device while anti-dengue IgM/IgG antibodies were detected by indirect ELISA. All NS1 positive samples were used for DENV serotyping using multiplex real-time PCR assay. Additionally, dengue samples were tested for CHIKV co-infection using IgM/IgG ELISA. A total of 6291 samples were collected among which 8.11% were NS1 positive while 2.5% were PCR positive. DENV-2 was the most common serotype (75.5%) detected, followed by DENV-1 in 16.1%, DENV-3 in 3.9% and DENV-4 in 0.7% while DENV-1 and DENV-4 concurrent infections were detected in 3.9% samples. DENV-1 was the predominant serotype (62.5%) detected from Lahore and Rawalpindi, while DENV-2 was the only serotype detected from Peshawar. Comorbidities resulted in a significant increase (p-value<0.001) in the duration of hospital stay of the patients. Type 2 diabetes mellitus substantially (p-value = 0.004) contributed to the severity of the disease. Among a total of 590 dengue positive samples, 11.8% were also positive for CHIKV co-infection. Co-circulation of multiple DENV serotypes and CHIKV infection in Pakistan is a worrisome situation demanding the urgent attention of the public health experts to strengthen vector surveillance.
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spelling pubmed-84918852021-10-06 Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study Raza, Faiz Ahmed Javed, Hasnain Khan, Muhammad Mujeeb Ullah, Obaid Fatima, Areeba Zaheer, Muhammad Mohsin, Saima Hasnain, Shahida Khalid, Ruqyya Salam, Arslan Ahmed PLoS Negl Trop Dis Research Article Dengue has become endemic in Pakistan with annual recurrence. A sudden increase in the dengue cases was reported from Rawalpindi in 2016, while an outbreak occurred for the first time in Peshawar in 2017. Therefore, a multi-center study was carried out to determine the circulating dengue virus (DENV) serotypes and Chikungunya virus (CHIKV) co-infection in Lahore, Rawalpindi, and Peshawar cities in 2016–18. A hospital-based cross-sectional study was carried out in Lahore and Rawalpindi in 2016–18, while a community-based study was carried out in Peshawar in 2017. The study participants were tested for dengue NS1 antigen using an immunochromatographic device while anti-dengue IgM/IgG antibodies were detected by indirect ELISA. All NS1 positive samples were used for DENV serotyping using multiplex real-time PCR assay. Additionally, dengue samples were tested for CHIKV co-infection using IgM/IgG ELISA. A total of 6291 samples were collected among which 8.11% were NS1 positive while 2.5% were PCR positive. DENV-2 was the most common serotype (75.5%) detected, followed by DENV-1 in 16.1%, DENV-3 in 3.9% and DENV-4 in 0.7% while DENV-1 and DENV-4 concurrent infections were detected in 3.9% samples. DENV-1 was the predominant serotype (62.5%) detected from Lahore and Rawalpindi, while DENV-2 was the only serotype detected from Peshawar. Comorbidities resulted in a significant increase (p-value<0.001) in the duration of hospital stay of the patients. Type 2 diabetes mellitus substantially (p-value = 0.004) contributed to the severity of the disease. Among a total of 590 dengue positive samples, 11.8% were also positive for CHIKV co-infection. Co-circulation of multiple DENV serotypes and CHIKV infection in Pakistan is a worrisome situation demanding the urgent attention of the public health experts to strengthen vector surveillance. Public Library of Science 2021-09-23 /pmc/articles/PMC8491885/ /pubmed/34555034 http://dx.doi.org/10.1371/journal.pntd.0009802 Text en © 2021 Raza et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Raza, Faiz Ahmed
Javed, Hasnain
Khan, Muhammad Mujeeb
Ullah, Obaid
Fatima, Areeba
Zaheer, Muhammad
Mohsin, Saima
Hasnain, Shahida
Khalid, Ruqyya
Salam, Arslan Ahmed
Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title_full Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title_fullStr Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title_full_unstemmed Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title_short Dengue and Chikungunya virus co-infection in major metropolitan cities of provinces of Punjab and Khyber Pakhtunkhwa: A multi-center study
title_sort dengue and chikungunya virus co-infection in major metropolitan cities of provinces of punjab and khyber pakhtunkhwa: a multi-center study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491885/
https://www.ncbi.nlm.nih.gov/pubmed/34555034
http://dx.doi.org/10.1371/journal.pntd.0009802
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