Cargando…

Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy

Visceral leishmaniasis (VL) caused by Leishmania infantum is endemic in the Mediterranean basin with most of the infected human patients remaining asymptomatic. Recently, the saurian-associated Leishmania tarentolae was detected in human blood donors and in sheltered dogs. The circulation of L. infa...

Descripción completa

Detalles Bibliográficos
Autores principales: Iatta, Roberta, Mendoza-Roldan, Jairo Alfonso, Latrofa, Maria Stefania, Cascio, Antonio, Brianti, Emanuele, Pombi, Marco, Gabrielli, Simona, Otranto, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491888/
https://www.ncbi.nlm.nih.gov/pubmed/34555036
http://dx.doi.org/10.1371/journal.pntd.0009817
_version_ 1784578819723624448
author Iatta, Roberta
Mendoza-Roldan, Jairo Alfonso
Latrofa, Maria Stefania
Cascio, Antonio
Brianti, Emanuele
Pombi, Marco
Gabrielli, Simona
Otranto, Domenico
author_facet Iatta, Roberta
Mendoza-Roldan, Jairo Alfonso
Latrofa, Maria Stefania
Cascio, Antonio
Brianti, Emanuele
Pombi, Marco
Gabrielli, Simona
Otranto, Domenico
author_sort Iatta, Roberta
collection PubMed
description Visceral leishmaniasis (VL) caused by Leishmania infantum is endemic in the Mediterranean basin with most of the infected human patients remaining asymptomatic. Recently, the saurian-associated Leishmania tarentolae was detected in human blood donors and in sheltered dogs. The circulation of L. infantum and L. tarentolae was investigated in humans, dogs and cats living in the Pelagie islands (Sicily, Italy) by multiple serological and molecular testing. Human serum samples (n = 346) were tested to assess the exposure to L. infantum by immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) and to L. tarentolae by IFAT. Meanwhile, sera from dogs (n = 149) and cats (n = 32) were tested for both Leishmania species by IFAT and all blood samples, including those of humans, by specific sets of real time-PCR for L. infantum and L. tarentolae. The agreement between serological tests performed for human samples, and between serological and molecular diagnostic techniques for both human and animal samples were also assessed. Overall, 41 human samples (11.8%, 95% CI: 8.9–15.7) were positive to L. infantum (5.2%, 95% CI: 3.3–8.1), L. tarentolae (5.2%, 95% CI: 3.3–8.1) and to both species (1.4%, 95% CI: 0.6–3.3) by serology and/or molecular tests. A good agreement among the serological tests was determined. Both Leishmania spp. were serologically and/or molecularly detected in 39.6% dogs and 43.7% cats. In addition to L. infantum, also L. tarentolae circulates in human and animal populations, raising relevant public health implications. Further studies should investigate the potential beneficial effects of L. tarentolae in the protection against L. infantum infection.
format Online
Article
Text
id pubmed-8491888
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-84918882021-10-06 Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy Iatta, Roberta Mendoza-Roldan, Jairo Alfonso Latrofa, Maria Stefania Cascio, Antonio Brianti, Emanuele Pombi, Marco Gabrielli, Simona Otranto, Domenico PLoS Negl Trop Dis Research Article Visceral leishmaniasis (VL) caused by Leishmania infantum is endemic in the Mediterranean basin with most of the infected human patients remaining asymptomatic. Recently, the saurian-associated Leishmania tarentolae was detected in human blood donors and in sheltered dogs. The circulation of L. infantum and L. tarentolae was investigated in humans, dogs and cats living in the Pelagie islands (Sicily, Italy) by multiple serological and molecular testing. Human serum samples (n = 346) were tested to assess the exposure to L. infantum by immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) and to L. tarentolae by IFAT. Meanwhile, sera from dogs (n = 149) and cats (n = 32) were tested for both Leishmania species by IFAT and all blood samples, including those of humans, by specific sets of real time-PCR for L. infantum and L. tarentolae. The agreement between serological tests performed for human samples, and between serological and molecular diagnostic techniques for both human and animal samples were also assessed. Overall, 41 human samples (11.8%, 95% CI: 8.9–15.7) were positive to L. infantum (5.2%, 95% CI: 3.3–8.1), L. tarentolae (5.2%, 95% CI: 3.3–8.1) and to both species (1.4%, 95% CI: 0.6–3.3) by serology and/or molecular tests. A good agreement among the serological tests was determined. Both Leishmania spp. were serologically and/or molecularly detected in 39.6% dogs and 43.7% cats. In addition to L. infantum, also L. tarentolae circulates in human and animal populations, raising relevant public health implications. Further studies should investigate the potential beneficial effects of L. tarentolae in the protection against L. infantum infection. Public Library of Science 2021-09-23 /pmc/articles/PMC8491888/ /pubmed/34555036 http://dx.doi.org/10.1371/journal.pntd.0009817 Text en © 2021 Iatta et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Iatta, Roberta
Mendoza-Roldan, Jairo Alfonso
Latrofa, Maria Stefania
Cascio, Antonio
Brianti, Emanuele
Pombi, Marco
Gabrielli, Simona
Otranto, Domenico
Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title_full Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title_fullStr Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title_full_unstemmed Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title_short Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy
title_sort leishmania tarentolae and leishmania infantum in humans, dogs and cats in the pelagie archipelago, southern italy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491888/
https://www.ncbi.nlm.nih.gov/pubmed/34555036
http://dx.doi.org/10.1371/journal.pntd.0009817
work_keys_str_mv AT iattaroberta leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT mendozaroldanjairoalfonso leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT latrofamariastefania leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT cascioantonio leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT briantiemanuele leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT pombimarco leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT gabriellisimona leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly
AT otrantodomenico leishmaniatarentolaeandleishmaniainfantuminhumansdogsandcatsinthepelagiearchipelagosouthernitaly