Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2

Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding...

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Autores principales: Huerga Encabo, Hector, Ulferts, Rachel, Sharma, Aneesh, Beale, Rupert, Bonnet, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492889/
https://www.ncbi.nlm.nih.gov/pubmed/34632411
http://dx.doi.org/10.1016/j.xpro.2021.100903
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author Huerga Encabo, Hector
Ulferts, Rachel
Sharma, Aneesh
Beale, Rupert
Bonnet, Dominique
author_facet Huerga Encabo, Hector
Ulferts, Rachel
Sharma, Aneesh
Beale, Rupert
Bonnet, Dominique
author_sort Huerga Encabo, Hector
collection PubMed
description Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021)
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spelling pubmed-84928892021-10-06 Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2 Huerga Encabo, Hector Ulferts, Rachel Sharma, Aneesh Beale, Rupert Bonnet, Dominique STAR Protoc Protocol Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021) Elsevier 2021-10-06 /pmc/articles/PMC8492889/ /pubmed/34632411 http://dx.doi.org/10.1016/j.xpro.2021.100903 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Huerga Encabo, Hector
Ulferts, Rachel
Sharma, Aneesh
Beale, Rupert
Bonnet, Dominique
Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_full Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_fullStr Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_full_unstemmed Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_short Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2
title_sort infecting human hematopoietic stem and progenitor cells with sars-cov-2
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492889/
https://www.ncbi.nlm.nih.gov/pubmed/34632411
http://dx.doi.org/10.1016/j.xpro.2021.100903
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