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PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma

Background: This presented study was aimed to evaluate the diagnostic and prognostic value of PD-L1(+)Neutrophils (PD-L1(+)NEUT) and neutrophil to lymphocyte ratio (NLR) based on our previous experience of Foxp3(+)Treg in transplantation. Methods: the NLR cutoff value of 1.79 was used to include 136...

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Autores principales: Zhou, Lin, Wang, Jing, Lyu, Shao-cheng, Pan, Li-chao, Shi, Xian-jie, Du, Guo-sheng, He, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493317/
https://www.ncbi.nlm.nih.gov/pubmed/34605709
http://dx.doi.org/10.1177/15330338211045820
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author Zhou, Lin
Wang, Jing
Lyu, Shao-cheng
Pan, Li-chao
Shi, Xian-jie
Du, Guo-sheng
He, Qiang
author_facet Zhou, Lin
Wang, Jing
Lyu, Shao-cheng
Pan, Li-chao
Shi, Xian-jie
Du, Guo-sheng
He, Qiang
author_sort Zhou, Lin
collection PubMed
description Background: This presented study was aimed to evaluate the diagnostic and prognostic value of PD-L1(+)Neutrophils (PD-L1(+)NEUT) and neutrophil to lymphocyte ratio (NLR) based on our previous experience of Foxp3(+)Treg in transplantation. Methods: the NLR cutoff value of 1.79 was used to include 136 cases from the 204 patients with hepatocellular carcinoma (HCC) confirmed by clinical pathology, which were divided into highly-moderately and poorly differentiated HCC groups. The expressions of PD-L1(+)NEUT and Foxp3(+)Treg in peripheral blood and cancer tissue were detected with flow cytometry, meanwhile, PD-L1 and Foxp3 expressed in carcinoma and para-carcinoma tissues were marked by immunohistochemistry. Survival rates, including overall survival and disease-free survival, were calculated by the Kaplan–Meier curve and evaluated with the log-rank test. Finally, Cox risk regression model was used to analyze the independent risk factors for prognostic survival. Results: The level of PD-L1(+)NEUT, Foxp3(+)Treg, and NLR in peripheral blood of patients with poorly differentiated HCC were significantly increased (all P < .001). Both PD-L1(+)NEUT and NLR were positively correlated with Foxp3(+)Treg (r = 0.479, P = .0017; r = 0.58, P < .0001). The level of PD-L1(+)NEUT and Foxp3(+)Treg as well as PD-L1 and Foxp3 in cancer tissue and patients with poorly differentiated HCC were obviously increased (all P < .01), respectively. Cox regression analysis indicated that PD-L1(+)NEUT, NLR, and Foxp3(+)Treg were independent risk factors for the prognosis (P = .000, .000, .006) with a RR and 95%CI of 2.704-(2.155-3.393), 3.139-(2.361-4.173), 1.409-(1.105-1.798), respectively. Conclusion: PD-L1(+)NEUT, NLR, and Foxp3(+)Treg are independent risk factors for prognosis which maybe new marker of lower survival benefits.
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spelling pubmed-84933172021-10-07 PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma Zhou, Lin Wang, Jing Lyu, Shao-cheng Pan, Li-chao Shi, Xian-jie Du, Guo-sheng He, Qiang Technol Cancer Res Treat Original Article Background: This presented study was aimed to evaluate the diagnostic and prognostic value of PD-L1(+)Neutrophils (PD-L1(+)NEUT) and neutrophil to lymphocyte ratio (NLR) based on our previous experience of Foxp3(+)Treg in transplantation. Methods: the NLR cutoff value of 1.79 was used to include 136 cases from the 204 patients with hepatocellular carcinoma (HCC) confirmed by clinical pathology, which were divided into highly-moderately and poorly differentiated HCC groups. The expressions of PD-L1(+)NEUT and Foxp3(+)Treg in peripheral blood and cancer tissue were detected with flow cytometry, meanwhile, PD-L1 and Foxp3 expressed in carcinoma and para-carcinoma tissues were marked by immunohistochemistry. Survival rates, including overall survival and disease-free survival, were calculated by the Kaplan–Meier curve and evaluated with the log-rank test. Finally, Cox risk regression model was used to analyze the independent risk factors for prognostic survival. Results: The level of PD-L1(+)NEUT, Foxp3(+)Treg, and NLR in peripheral blood of patients with poorly differentiated HCC were significantly increased (all P < .001). Both PD-L1(+)NEUT and NLR were positively correlated with Foxp3(+)Treg (r = 0.479, P = .0017; r = 0.58, P < .0001). The level of PD-L1(+)NEUT and Foxp3(+)Treg as well as PD-L1 and Foxp3 in cancer tissue and patients with poorly differentiated HCC were obviously increased (all P < .01), respectively. Cox regression analysis indicated that PD-L1(+)NEUT, NLR, and Foxp3(+)Treg were independent risk factors for the prognosis (P = .000, .000, .006) with a RR and 95%CI of 2.704-(2.155-3.393), 3.139-(2.361-4.173), 1.409-(1.105-1.798), respectively. Conclusion: PD-L1(+)NEUT, NLR, and Foxp3(+)Treg are independent risk factors for prognosis which maybe new marker of lower survival benefits. SAGE Publications 2021-10-04 /pmc/articles/PMC8493317/ /pubmed/34605709 http://dx.doi.org/10.1177/15330338211045820 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Zhou, Lin
Wang, Jing
Lyu, Shao-cheng
Pan, Li-chao
Shi, Xian-jie
Du, Guo-sheng
He, Qiang
PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title_full PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title_fullStr PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title_full_unstemmed PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title_short PD-L1(+)NEUT, Foxp3(+)Treg, and NLR as New Prognostic Marker with Low Survival Benefits Value in Hepatocellular Carcinoma
title_sort pd-l1(+)neut, foxp3(+)treg, and nlr as new prognostic marker with low survival benefits value in hepatocellular carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493317/
https://www.ncbi.nlm.nih.gov/pubmed/34605709
http://dx.doi.org/10.1177/15330338211045820
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