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SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen
[Image: see text] Introduction: A new microfluidic-based method with electrochemical detection was developed for the simultaneous quantification of acetaminophen (AP) and phenylephrine (PHE) pharmaceuticals in the human blood and pharmaceuticals (e.g. tablet and drop). Methods: The separation was ac...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences (TUOMS Publishing Group)
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494256/ https://www.ncbi.nlm.nih.gov/pubmed/34631488 http://dx.doi.org/10.34172/bi.2021.35 |
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author | Abdollahi-Aghdam, Abdollah Majidi, Mir Reza Veladi, Hadi Omidi, Yadollah |
author_facet | Abdollahi-Aghdam, Abdollah Majidi, Mir Reza Veladi, Hadi Omidi, Yadollah |
author_sort | Abdollahi-Aghdam, Abdollah |
collection | PubMed |
description | [Image: see text] Introduction: A new microfluidic-based method with electrochemical detection was developed for the simultaneous quantification of acetaminophen (AP) and phenylephrine (PHE) pharmaceuticals in the human blood and pharmaceuticals (e.g. tablet and drop). Methods: The separation was achieved on a SU8/glass microchip with a 100 µm Pt working electrode that was positioned out of the channel and 2-(N-morpholino) ethanesulfonic acid was used as a running buffer (pH 7, 10 mM). Home designed modulated high voltage power supply and dual time switcher was used for controlling the injection and separation of the analytes in the unpinched injection mode. Results: The injection was carried out using +750 V for 7 seconds, and the separation and detection voltages were set at +1000 V and +0.9 V, respectively. Critical parameters such as detection potential, buffer concentration, injection, and separation voltage were studied in terms of their effects on the resolution, peak height, and migration times. For each analyte, the correlation coefficients were over 0.99 (n = 6). The developed microchip was able to detect AP and phenylephrine simultaneously with the limit of detection of 7.9 and 5.2 (µg/mL) respectively for PHE and AP and excellent linear range of 10-200 (µg/mL). The recovery of the drugs ranged from 96% to 103%, while the repeatability of the method through inter- and intra-day was lower than 7%. Conclusion: The developed method offers several advantages, including easy sample pretreatment process, simplicity, very fast analysis compared to other typical chromatographic methods. Thus, the proposed microfluidic-based method is proposed to be used as a time- and cost-effective monitoring method for the analysis of AP and PHE. |
format | Online Article Text |
id | pubmed-8494256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Tabriz University of Medical Sciences (TUOMS Publishing Group) |
record_format | MEDLINE/PubMed |
spelling | pubmed-84942562021-10-08 SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen Abdollahi-Aghdam, Abdollah Majidi, Mir Reza Veladi, Hadi Omidi, Yadollah Bioimpacts Original Research [Image: see text] Introduction: A new microfluidic-based method with electrochemical detection was developed for the simultaneous quantification of acetaminophen (AP) and phenylephrine (PHE) pharmaceuticals in the human blood and pharmaceuticals (e.g. tablet and drop). Methods: The separation was achieved on a SU8/glass microchip with a 100 µm Pt working electrode that was positioned out of the channel and 2-(N-morpholino) ethanesulfonic acid was used as a running buffer (pH 7, 10 mM). Home designed modulated high voltage power supply and dual time switcher was used for controlling the injection and separation of the analytes in the unpinched injection mode. Results: The injection was carried out using +750 V for 7 seconds, and the separation and detection voltages were set at +1000 V and +0.9 V, respectively. Critical parameters such as detection potential, buffer concentration, injection, and separation voltage were studied in terms of their effects on the resolution, peak height, and migration times. For each analyte, the correlation coefficients were over 0.99 (n = 6). The developed microchip was able to detect AP and phenylephrine simultaneously with the limit of detection of 7.9 and 5.2 (µg/mL) respectively for PHE and AP and excellent linear range of 10-200 (µg/mL). The recovery of the drugs ranged from 96% to 103%, while the repeatability of the method through inter- and intra-day was lower than 7%. Conclusion: The developed method offers several advantages, including easy sample pretreatment process, simplicity, very fast analysis compared to other typical chromatographic methods. Thus, the proposed microfluidic-based method is proposed to be used as a time- and cost-effective monitoring method for the analysis of AP and PHE. Tabriz University of Medical Sciences (TUOMS Publishing Group) 2021 2020-01-10 /pmc/articles/PMC8494256/ /pubmed/34631488 http://dx.doi.org/10.34172/bi.2021.35 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc/4.0/ This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Research Abdollahi-Aghdam, Abdollah Majidi, Mir Reza Veladi, Hadi Omidi, Yadollah SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title | SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title_full | SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title_fullStr | SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title_full_unstemmed | SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title_short | SU8/glass microchip capillary electrophoresis integrated with Pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
title_sort | su8/glass microchip capillary electrophoresis integrated with pt electrodes for separation and simultaneous detection of phenylephrine and acetaminophen |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494256/ https://www.ncbi.nlm.nih.gov/pubmed/34631488 http://dx.doi.org/10.34172/bi.2021.35 |
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