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Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity
Supplementation with precursors of NAD has been shown to prevent and reverse insulin resistance, mitochondrial dysfunction, and liver damage in mouse models of diet-induced obesity. We asked whether the beneficial effects of supplementation with the NAD precursor nicotinamide riboside (NR) are depen...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bioscientifica Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494379/ https://www.ncbi.nlm.nih.gov/pubmed/34370682 http://dx.doi.org/10.1530/JOE-21-0123 |
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author | Cartwright, David M Oakey, Lucy A Fletcher, Rachel S Doig, Craig L Heising, Silke Larner, Dean P Nasteska, Daniela Berry, Caitlin E Heaselgrave, Sam R Ludwig, Christian Hodson, David J Lavery, Gareth G Garten, Antje |
author_facet | Cartwright, David M Oakey, Lucy A Fletcher, Rachel S Doig, Craig L Heising, Silke Larner, Dean P Nasteska, Daniela Berry, Caitlin E Heaselgrave, Sam R Ludwig, Christian Hodson, David J Lavery, Gareth G Garten, Antje |
author_sort | Cartwright, David M |
collection | PubMed |
description | Supplementation with precursors of NAD has been shown to prevent and reverse insulin resistance, mitochondrial dysfunction, and liver damage in mouse models of diet-induced obesity. We asked whether the beneficial effects of supplementation with the NAD precursor nicotinamide riboside (NR) are dependent on mouse strain. We compared the effects of NR supplementation on whole-body energy metabolism and mitochondrial function in mildly obese C57BL/6N and C57BL/6J mice, two commonly used strains to investigate metabolism. Male C57BL/6N and C57BL/6J mice were fed a high-fat diet (HFD) or standard chow with or without NR supplementation for 8 weeks. Body and organ weights, glucose tolerance, and metabolic parameters as well as mitochondrial O(2) flux in liver and muscle fibers were assessed. We found that NR supplementation had no influence on body or organ weight, glucose metabolism or hepatic lipid accumulation, energy expenditure, or metabolic flexibility but increased mitochondrial respiration in soleus muscle in both mouse strains. Strain-dependent differences were detected for body and fat depot weight, fasting blood glucose, hepatic lipid accumulation, and energy expenditure. We conclude that, in mild obesity, NR supplementation does not alter metabolic phenotype in two commonly used laboratory mouse strains. |
format | Online Article Text |
id | pubmed-8494379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Bioscientifica Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-84943792021-10-08 Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity Cartwright, David M Oakey, Lucy A Fletcher, Rachel S Doig, Craig L Heising, Silke Larner, Dean P Nasteska, Daniela Berry, Caitlin E Heaselgrave, Sam R Ludwig, Christian Hodson, David J Lavery, Gareth G Garten, Antje J Endocrinol Research Supplementation with precursors of NAD has been shown to prevent and reverse insulin resistance, mitochondrial dysfunction, and liver damage in mouse models of diet-induced obesity. We asked whether the beneficial effects of supplementation with the NAD precursor nicotinamide riboside (NR) are dependent on mouse strain. We compared the effects of NR supplementation on whole-body energy metabolism and mitochondrial function in mildly obese C57BL/6N and C57BL/6J mice, two commonly used strains to investigate metabolism. Male C57BL/6N and C57BL/6J mice were fed a high-fat diet (HFD) or standard chow with or without NR supplementation for 8 weeks. Body and organ weights, glucose tolerance, and metabolic parameters as well as mitochondrial O(2) flux in liver and muscle fibers were assessed. We found that NR supplementation had no influence on body or organ weight, glucose metabolism or hepatic lipid accumulation, energy expenditure, or metabolic flexibility but increased mitochondrial respiration in soleus muscle in both mouse strains. Strain-dependent differences were detected for body and fat depot weight, fasting blood glucose, hepatic lipid accumulation, and energy expenditure. We conclude that, in mild obesity, NR supplementation does not alter metabolic phenotype in two commonly used laboratory mouse strains. Bioscientifica Ltd 2021-08-09 /pmc/articles/PMC8494379/ /pubmed/34370682 http://dx.doi.org/10.1530/JOE-21-0123 Text en © The authors https://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Cartwright, David M Oakey, Lucy A Fletcher, Rachel S Doig, Craig L Heising, Silke Larner, Dean P Nasteska, Daniela Berry, Caitlin E Heaselgrave, Sam R Ludwig, Christian Hodson, David J Lavery, Gareth G Garten, Antje Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title | Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title_full | Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title_fullStr | Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title_full_unstemmed | Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title_short | Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
title_sort | nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494379/ https://www.ncbi.nlm.nih.gov/pubmed/34370682 http://dx.doi.org/10.1530/JOE-21-0123 |
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