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Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer

Objective: In this study, we mainly explored two questions: Which microorganisms were functionally active in the endometrium of patients with endometrial cancer (EC)? What kind of response did the human host respond to functionally active microorganisms? Methods: Nine endometrial cancer patients and...

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Autores principales: Chen, Peigen, Guo, Yingchun, Jia, Lei, Wan, Jing, He, TianTian, Fang, Cong, Li, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495019/
https://www.ncbi.nlm.nih.gov/pubmed/34631710
http://dx.doi.org/10.3389/fcell.2021.727286
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author Chen, Peigen
Guo, Yingchun
Jia, Lei
Wan, Jing
He, TianTian
Fang, Cong
Li, Tingting
author_facet Chen, Peigen
Guo, Yingchun
Jia, Lei
Wan, Jing
He, TianTian
Fang, Cong
Li, Tingting
author_sort Chen, Peigen
collection PubMed
description Objective: In this study, we mainly explored two questions: Which microorganisms were functionally active in the endometrium of patients with endometrial cancer (EC)? What kind of response did the human host respond to functionally active microorganisms? Methods: Nine endometrial cancer patients and eight normal subjects were included in this study. HMP Unified Metabolic Analysis Network 3 (HUMAnN3) was used to obtain functional information of microorganisms. In addition, metaCyc-based GSEA functional enrichment analysis was used to obtain information on the metabolic pathways of the human host. At the same time, the O2PLS model and Spearman correlation analysis were used to analyze the microorganisms–host interaction. Results: With the novel metatranscriptome analysis pipeline, we described the composition of more than 5,000 functionally active microorganisms and analyzed the difference in microorganisms between the EC and the normal group. Our research found that these microorganisms were involved in part of the metabolic process of endometrial cancer, such as 6-sulfo-sialyl Lewis x epitope, N-acetyl-beta-glucosaminyl. In addition, the host–microbiota crosstalk of EC endometrium also included many biological processes, mainly functions related to tumor migration and the Apelin signaling pathway. Conclusion: The functionally active microorganisms in the EC endometrium played an essential role in the occurrence and migration of tumors. This meant that functionally active microorganisms could not be ignored in the treatment of endometrial cancer. This study helped to better understand the possible role of endometrial functional, active microorganisms in the occurrence and development of EC in patients with endometrial cancer and provided new information for new attempts to treat EC.
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spelling pubmed-84950192021-10-08 Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer Chen, Peigen Guo, Yingchun Jia, Lei Wan, Jing He, TianTian Fang, Cong Li, Tingting Front Cell Dev Biol Cell and Developmental Biology Objective: In this study, we mainly explored two questions: Which microorganisms were functionally active in the endometrium of patients with endometrial cancer (EC)? What kind of response did the human host respond to functionally active microorganisms? Methods: Nine endometrial cancer patients and eight normal subjects were included in this study. HMP Unified Metabolic Analysis Network 3 (HUMAnN3) was used to obtain functional information of microorganisms. In addition, metaCyc-based GSEA functional enrichment analysis was used to obtain information on the metabolic pathways of the human host. At the same time, the O2PLS model and Spearman correlation analysis were used to analyze the microorganisms–host interaction. Results: With the novel metatranscriptome analysis pipeline, we described the composition of more than 5,000 functionally active microorganisms and analyzed the difference in microorganisms between the EC and the normal group. Our research found that these microorganisms were involved in part of the metabolic process of endometrial cancer, such as 6-sulfo-sialyl Lewis x epitope, N-acetyl-beta-glucosaminyl. In addition, the host–microbiota crosstalk of EC endometrium also included many biological processes, mainly functions related to tumor migration and the Apelin signaling pathway. Conclusion: The functionally active microorganisms in the EC endometrium played an essential role in the occurrence and migration of tumors. This meant that functionally active microorganisms could not be ignored in the treatment of endometrial cancer. This study helped to better understand the possible role of endometrial functional, active microorganisms in the occurrence and development of EC in patients with endometrial cancer and provided new information for new attempts to treat EC. Frontiers Media S.A. 2021-09-23 /pmc/articles/PMC8495019/ /pubmed/34631710 http://dx.doi.org/10.3389/fcell.2021.727286 Text en Copyright © 2021 Chen, Guo, Jia, Wan, He, Fang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chen, Peigen
Guo, Yingchun
Jia, Lei
Wan, Jing
He, TianTian
Fang, Cong
Li, Tingting
Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title_full Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title_fullStr Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title_full_unstemmed Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title_short Interaction Between Functionally Activate Endometrial Microbiota and Host Gene Regulation in Endometrial Cancer
title_sort interaction between functionally activate endometrial microbiota and host gene regulation in endometrial cancer
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495019/
https://www.ncbi.nlm.nih.gov/pubmed/34631710
http://dx.doi.org/10.3389/fcell.2021.727286
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