Cargando…

Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.

Cherry laurel (Prunus laurocerasus L.) is an extreme polyploid (2n = 22x) species of the Rosaceae family where gametophytic self-incompatibility (GSI) prevents inbreeding. This study was carried out to identify the S-ribonuclease alleles (S-RNases) of P. laurocerasus using PCR amplification of the f...

Descripción completa

Detalles Bibliográficos
Autores principales: Halász, Júlia, Molnár, Anna Borbála, Ilhan, Gulce, Ercisli, Sezai, Hegedűs, Attila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495262/
https://www.ncbi.nlm.nih.gov/pubmed/34630463
http://dx.doi.org/10.3389/fpls.2021.715414
_version_ 1784579506513641472
author Halász, Júlia
Molnár, Anna Borbála
Ilhan, Gulce
Ercisli, Sezai
Hegedűs, Attila
author_facet Halász, Júlia
Molnár, Anna Borbála
Ilhan, Gulce
Ercisli, Sezai
Hegedűs, Attila
author_sort Halász, Júlia
collection PubMed
description Cherry laurel (Prunus laurocerasus L.) is an extreme polyploid (2n = 22x) species of the Rosaceae family where gametophytic self-incompatibility (GSI) prevents inbreeding. This study was carried out to identify the S-ribonuclease alleles (S-RNases) of P. laurocerasus using PCR amplification of the first and second intron region of the S-RNase gene, cloning and sequencing. A total of 23 putative S-RNase alleles (S(1)–S(20), S(5)(m), S(13)(m), and S(18)(m)) were sequenced from the second (C2) to the fifth conserved region (C5), and they shared significant homology to other Prunus S-RNases. The length of the sequenced amplicons ranged from 505 to 1,544 bp, and similar sizes prevented the proper discrimination of some alleles based on PCR analysis. We have found three putatively non-functional alleles (S(5)(m), S(18)(m), and S(9)) coding for truncated proteins. Although firm conclusions cannot be drawn, our data seem to support that heteroallelic pollen cannot induce self-compatibility in this polyploid Prunus species. The identities in the deduced amino acid sequences between the P. laurocerasus and other Prunus S-RNases ranged between 44 and 100%, without a discontinuity gap separating the identity percentages of trans-specific and more distantly related alleles. The phylogenetic position, the identities in nucleotide sequences of the second intron and in deduced amino acid sequences found one or more trans-specific alleles for all but S(10), S(14), S(18), and S(20) cherry laurel RNases. The analysis of mutational frequencies in trans-specific allele pairs indicated the region RC4–C5 accepts the most amino acid replacements and hence it may contribute to allele-specificity. Our results form the basis of future studies to confirm the existence and function of the GSI system in this extreme polyploid species and the alleles identified will be also useful for phylogenetic studies of Prunus S-RNases as the number of S-RNase sequences was limited in the Racemose group of Prunus (where P. laurocerasus belongs to).
format Online
Article
Text
id pubmed-8495262
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84952622021-10-08 Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L. Halász, Júlia Molnár, Anna Borbála Ilhan, Gulce Ercisli, Sezai Hegedűs, Attila Front Plant Sci Plant Science Cherry laurel (Prunus laurocerasus L.) is an extreme polyploid (2n = 22x) species of the Rosaceae family where gametophytic self-incompatibility (GSI) prevents inbreeding. This study was carried out to identify the S-ribonuclease alleles (S-RNases) of P. laurocerasus using PCR amplification of the first and second intron region of the S-RNase gene, cloning and sequencing. A total of 23 putative S-RNase alleles (S(1)–S(20), S(5)(m), S(13)(m), and S(18)(m)) were sequenced from the second (C2) to the fifth conserved region (C5), and they shared significant homology to other Prunus S-RNases. The length of the sequenced amplicons ranged from 505 to 1,544 bp, and similar sizes prevented the proper discrimination of some alleles based on PCR analysis. We have found three putatively non-functional alleles (S(5)(m), S(18)(m), and S(9)) coding for truncated proteins. Although firm conclusions cannot be drawn, our data seem to support that heteroallelic pollen cannot induce self-compatibility in this polyploid Prunus species. The identities in the deduced amino acid sequences between the P. laurocerasus and other Prunus S-RNases ranged between 44 and 100%, without a discontinuity gap separating the identity percentages of trans-specific and more distantly related alleles. The phylogenetic position, the identities in nucleotide sequences of the second intron and in deduced amino acid sequences found one or more trans-specific alleles for all but S(10), S(14), S(18), and S(20) cherry laurel RNases. The analysis of mutational frequencies in trans-specific allele pairs indicated the region RC4–C5 accepts the most amino acid replacements and hence it may contribute to allele-specificity. Our results form the basis of future studies to confirm the existence and function of the GSI system in this extreme polyploid species and the alleles identified will be also useful for phylogenetic studies of Prunus S-RNases as the number of S-RNase sequences was limited in the Racemose group of Prunus (where P. laurocerasus belongs to). Frontiers Media S.A. 2021-09-23 /pmc/articles/PMC8495262/ /pubmed/34630463 http://dx.doi.org/10.3389/fpls.2021.715414 Text en Copyright © 2021 Halász, Molnár, Ilhan, Ercisli and Hegedűs. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Halász, Júlia
Molnár, Anna Borbála
Ilhan, Gulce
Ercisli, Sezai
Hegedűs, Attila
Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title_full Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title_fullStr Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title_full_unstemmed Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title_short Identification and Molecular Analysis of Putative Self-Incompatibility Ribonuclease Alleles in an Extreme Polyploid Species, Prunus laurocerasus L.
title_sort identification and molecular analysis of putative self-incompatibility ribonuclease alleles in an extreme polyploid species, prunus laurocerasus l.
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495262/
https://www.ncbi.nlm.nih.gov/pubmed/34630463
http://dx.doi.org/10.3389/fpls.2021.715414
work_keys_str_mv AT halaszjulia identificationandmolecularanalysisofputativeselfincompatibilityribonucleaseallelesinanextremepolyploidspeciesprunuslaurocerasusl
AT molnarannaborbala identificationandmolecularanalysisofputativeselfincompatibilityribonucleaseallelesinanextremepolyploidspeciesprunuslaurocerasusl
AT ilhangulce identificationandmolecularanalysisofputativeselfincompatibilityribonucleaseallelesinanextremepolyploidspeciesprunuslaurocerasusl
AT ercislisezai identificationandmolecularanalysisofputativeselfincompatibilityribonucleaseallelesinanextremepolyploidspeciesprunuslaurocerasusl
AT hegedusattila identificationandmolecularanalysisofputativeselfincompatibilityribonucleaseallelesinanextremepolyploidspeciesprunuslaurocerasusl