A quantitative method for detecting meat contamination based on specific polypeptides

OBJECTIVE: This study was aimed to establish a quantitative detection method for meat contamination based on specific polypeptides. METHODS: Thermally stable peptides with good responses were screened by high resolution liquid chromatography tandem mass spectrometry. Standard curves of specific polypeptide were established by triple quadrupole mass spectrometry. Finally, the adulteration of commercial samples was detected according to the standard curve. RESULTS: Fifteen thermally stable peptides with good responses were screened. The selected specific peptides can be detected stably in raw meat and deep processed meat with the detection limit up to 1% and have a good linear relationship with the corresponding muscle composition. CONCLUSION: This method can be effectively used for quantitative analysis of commercial samples.