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Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows

OBJECTIVE: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding mate...

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Autores principales: Li, Han, Wang, Xiangming, Wu, Yan, Zhang, Dingran, Xu, Hongyang, Xu, Hongrun, Xing, Xiaoguang, Qi, Zhili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Animal Bioscience 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495337/
https://www.ncbi.nlm.nih.gov/pubmed/33171032
http://dx.doi.org/10.5713/ajas.20.0565
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author Li, Han
Wang, Xiangming
Wu, Yan
Zhang, Dingran
Xu, Hongyang
Xu, Hongrun
Xing, Xiaoguang
Qi, Zhili
author_facet Li, Han
Wang, Xiangming
Wu, Yan
Zhang, Dingran
Xu, Hongyang
Xu, Hongrun
Xing, Xiaoguang
Qi, Zhili
author_sort Li, Han
collection PubMed
description OBJECTIVE: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. METHODS: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. RESULTS: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). CONCLUSION: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.
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spelling pubmed-84953372021-10-08 Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows Li, Han Wang, Xiangming Wu, Yan Zhang, Dingran Xu, Hongyang Xu, Hongrun Xing, Xiaoguang Qi, Zhili Anim Biosci Article OBJECTIVE: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. METHODS: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. RESULTS: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). CONCLUSION: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows. Animal Bioscience 2021-09 2020-11-03 /pmc/articles/PMC8495337/ /pubmed/33171032 http://dx.doi.org/10.5713/ajas.20.0565 Text en Copyright © 2021 by Animal Bioscience https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Li, Han
Wang, Xiangming
Wu, Yan
Zhang, Dingran
Xu, Hongyang
Xu, Hongrun
Xing, Xiaoguang
Qi, Zhili
Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title_full Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title_fullStr Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title_full_unstemmed Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title_short Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
title_sort relationships among bedding materials, bedding bacterial composition and lameness in dairy cows
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495337/
https://www.ncbi.nlm.nih.gov/pubmed/33171032
http://dx.doi.org/10.5713/ajas.20.0565
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