The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly

Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complem...

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Autores principales: Chen, Minghai, Yan, Chuang, Qin, Fujun, Zheng, Luping, Zhang, Xian-En
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495407/
https://www.ncbi.nlm.nih.gov/pubmed/34671206
http://dx.doi.org/10.7150/ijbs.64977
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author Chen, Minghai
Yan, Chuang
Qin, Fujun
Zheng, Luping
Zhang, Xian-En
author_facet Chen, Minghai
Yan, Chuang
Qin, Fujun
Zheng, Luping
Zhang, Xian-En
author_sort Chen, Minghai
collection PubMed
description Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins of SARS-CoV-2, and also specifically interacted with the putative packaging signal (PS) of SARS-CoV-2. We also demonstrated that the PS core containing PS576 RNA bears a functional PS, important for the assembly of the viral RNA into virus like particles (VLPs), and the packaging of SARS-CoV-2 RNA was N dependent.
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spelling pubmed-84954072021-10-19 The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly Chen, Minghai Yan, Chuang Qin, Fujun Zheng, Luping Zhang, Xian-En Int J Biol Sci Research Paper Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins of SARS-CoV-2, and also specifically interacted with the putative packaging signal (PS) of SARS-CoV-2. We also demonstrated that the PS core containing PS576 RNA bears a functional PS, important for the assembly of the viral RNA into virus like particles (VLPs), and the packaging of SARS-CoV-2 RNA was N dependent. Ivyspring International Publisher 2021-09-07 /pmc/articles/PMC8495407/ /pubmed/34671206 http://dx.doi.org/10.7150/ijbs.64977 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chen, Minghai
Yan, Chuang
Qin, Fujun
Zheng, Luping
Zhang, Xian-En
The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title_full The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title_fullStr The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title_full_unstemmed The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title_short The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly
title_sort intraviral protein-protein interaction of sars-cov-2 reveals the key role of n protein in virus-like particle assembly
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495407/
https://www.ncbi.nlm.nih.gov/pubmed/34671206
http://dx.doi.org/10.7150/ijbs.64977
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