Cargando…

Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations

Cardiovascular diseases (CVDs) are a group of disorders of the blood vessels and heart, which are considered as the leading causes of death worldwide. The pathology of CVDs could be related to the functional abnormalities of multiple cell types in the heart. Single-cell RNA sequencing (scRNA-seq) te...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Mingqiang, Gu, Mingxia, Liu, Ling, Liu, Yu, Tian, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495612/
https://www.ncbi.nlm.nih.gov/pubmed/34629873
http://dx.doi.org/10.2147/VHRM.S288090
_version_ 1784579587876847616
author Wang, Mingqiang
Gu, Mingxia
Liu, Ling
Liu, Yu
Tian, Lei
author_facet Wang, Mingqiang
Gu, Mingxia
Liu, Ling
Liu, Yu
Tian, Lei
author_sort Wang, Mingqiang
collection PubMed
description Cardiovascular diseases (CVDs) are a group of disorders of the blood vessels and heart, which are considered as the leading causes of death worldwide. The pathology of CVDs could be related to the functional abnormalities of multiple cell types in the heart. Single-cell RNA sequencing (scRNA-seq) technology is a powerful method for characterizing individual cells and elucidating the molecular mechanisms by providing a high resolution of transcriptomic changes at the single-cell level. Specifically, scRNA-seq has provided novel insights into CVDs by identifying rare cardiac cell types, inferring the trajectory tree, estimating RNA velocity, elucidating the cell–cell communication, and comparing healthy and pathological heart samples. In this review, we summarize the different scRNA-seq platforms and published single-cell datasets in the cardiovascular field, and describe the utilities and limitations of this technology. Lastly, we discuss the future perspective of the application of scRNA-seq technology into cardiovascular research.
format Online
Article
Text
id pubmed-8495612
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-84956122021-10-08 Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations Wang, Mingqiang Gu, Mingxia Liu, Ling Liu, Yu Tian, Lei Vasc Health Risk Manag Review Cardiovascular diseases (CVDs) are a group of disorders of the blood vessels and heart, which are considered as the leading causes of death worldwide. The pathology of CVDs could be related to the functional abnormalities of multiple cell types in the heart. Single-cell RNA sequencing (scRNA-seq) technology is a powerful method for characterizing individual cells and elucidating the molecular mechanisms by providing a high resolution of transcriptomic changes at the single-cell level. Specifically, scRNA-seq has provided novel insights into CVDs by identifying rare cardiac cell types, inferring the trajectory tree, estimating RNA velocity, elucidating the cell–cell communication, and comparing healthy and pathological heart samples. In this review, we summarize the different scRNA-seq platforms and published single-cell datasets in the cardiovascular field, and describe the utilities and limitations of this technology. Lastly, we discuss the future perspective of the application of scRNA-seq technology into cardiovascular research. Dove 2021-10-02 /pmc/articles/PMC8495612/ /pubmed/34629873 http://dx.doi.org/10.2147/VHRM.S288090 Text en © 2021 Wang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Review
Wang, Mingqiang
Gu, Mingxia
Liu, Ling
Liu, Yu
Tian, Lei
Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title_full Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title_fullStr Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title_full_unstemmed Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title_short Single-Cell RNA Sequencing (scRNA-seq) in Cardiac Tissue: Applications and Limitations
title_sort single-cell rna sequencing (scrna-seq) in cardiac tissue: applications and limitations
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495612/
https://www.ncbi.nlm.nih.gov/pubmed/34629873
http://dx.doi.org/10.2147/VHRM.S288090
work_keys_str_mv AT wangmingqiang singlecellrnasequencingscrnaseqincardiactissueapplicationsandlimitations
AT gumingxia singlecellrnasequencingscrnaseqincardiactissueapplicationsandlimitations
AT liuling singlecellrnasequencingscrnaseqincardiactissueapplicationsandlimitations
AT liuyu singlecellrnasequencingscrnaseqincardiactissueapplicationsandlimitations
AT tianlei singlecellrnasequencingscrnaseqincardiactissueapplicationsandlimitations