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Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System

INTRODUCTION: The current study aimed to assess the interference of in vitro haemolysis on complete blood count (CBC) using Abbott Alinity hq system, and to determine which haemolysis levels affect the reliability of sample results. MATERIALS AND METHODS: Blood samples obtained from 25 volunteers in...

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Autores principales: Ercan, Müjgan, Akbulut, Emiş Deniz, Bayraktar, Nihayet, Ercan, Şerif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Society of Medical Biochemistry and Laboratory Medicine 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495619/
https://www.ncbi.nlm.nih.gov/pubmed/34658647
http://dx.doi.org/10.11613/BM.2021.030706
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author Ercan, Müjgan
Akbulut, Emiş Deniz
Bayraktar, Nihayet
Ercan, Şerif
author_facet Ercan, Müjgan
Akbulut, Emiş Deniz
Bayraktar, Nihayet
Ercan, Şerif
author_sort Ercan, Müjgan
collection PubMed
description INTRODUCTION: The current study aimed to assess the interference of in vitro haemolysis on complete blood count (CBC) using Abbott Alinity hq system, and to determine which haemolysis levels affect the reliability of sample results. MATERIALS AND METHODS: Blood samples obtained from 25 volunteers in K3-EDTA tubes were divided into four aliquots. The first aliquot was not subjected to any intervention. The second, third and fourth aliquots were passed through a fine needle 2, 4 and 6 times, respectively. Complete blood count was performed by multi-angle polarized scatter separation technology and haemolysis index (HI) was assessed from the plasma samples separated by centrifugation. Five groups were formed according to the HI values. The percentage biases between the results of non-haemolysed and haemolysed groups were compared with the desirable bias limits from The European Federation of Clinical Chemistry and Laboratory Medicine database and reference change values (RCVs). RESULTS: In groups 1 to 4, the effects of haemolysis on CBC parameters were acceptable comparing to the analytical bias except for lymphocytes (7.26%-7.42%), MCH (2.59%), and MCHC (0.47%-2.81%). Results of group 5 (gross haemolysis) showed decreases in HCT(- 4.56%), RBC (- 4.07%) count and increase in lymphocyte (11.60%) count higher than the analytical performance specifications. Moreover, variations in MCH (4.65%) and MCHC (5.24%) were exceeding the RCVs. CONCLUSIONS: Gross haemolysis (haemoglobin concentration > 10 g/L) is likely to produce unreliable CBC results on non-pathological samples. Further studies including pathological specimens are needed.
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spelling pubmed-84956192021-10-15 Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System Ercan, Müjgan Akbulut, Emiş Deniz Bayraktar, Nihayet Ercan, Şerif Biochem Med (Zagreb) Original Articles INTRODUCTION: The current study aimed to assess the interference of in vitro haemolysis on complete blood count (CBC) using Abbott Alinity hq system, and to determine which haemolysis levels affect the reliability of sample results. MATERIALS AND METHODS: Blood samples obtained from 25 volunteers in K3-EDTA tubes were divided into four aliquots. The first aliquot was not subjected to any intervention. The second, third and fourth aliquots were passed through a fine needle 2, 4 and 6 times, respectively. Complete blood count was performed by multi-angle polarized scatter separation technology and haemolysis index (HI) was assessed from the plasma samples separated by centrifugation. Five groups were formed according to the HI values. The percentage biases between the results of non-haemolysed and haemolysed groups were compared with the desirable bias limits from The European Federation of Clinical Chemistry and Laboratory Medicine database and reference change values (RCVs). RESULTS: In groups 1 to 4, the effects of haemolysis on CBC parameters were acceptable comparing to the analytical bias except for lymphocytes (7.26%-7.42%), MCH (2.59%), and MCHC (0.47%-2.81%). Results of group 5 (gross haemolysis) showed decreases in HCT(- 4.56%), RBC (- 4.07%) count and increase in lymphocyte (11.60%) count higher than the analytical performance specifications. Moreover, variations in MCH (4.65%) and MCHC (5.24%) were exceeding the RCVs. CONCLUSIONS: Gross haemolysis (haemoglobin concentration > 10 g/L) is likely to produce unreliable CBC results on non-pathological samples. Further studies including pathological specimens are needed. Croatian Society of Medical Biochemistry and Laboratory Medicine 2021-10-15 2021-10-15 /pmc/articles/PMC8495619/ /pubmed/34658647 http://dx.doi.org/10.11613/BM.2021.030706 Text en Croatian Society of Medical Biochemistry and Laboratory Medicine. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ercan, Müjgan
Akbulut, Emiş Deniz
Bayraktar, Nihayet
Ercan, Şerif
Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title_full Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title_fullStr Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title_full_unstemmed Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title_short Effects of specimen haemolysis on complete blood count results by Abbott Alinity hq System
title_sort effects of specimen haemolysis on complete blood count results by abbott alinity hq system
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495619/
https://www.ncbi.nlm.nih.gov/pubmed/34658647
http://dx.doi.org/10.11613/BM.2021.030706
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