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A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries

A recent and powerful technique is to obtain transcriptomes from rare cell populations, such as single neurons in Caenorhabditis elegans, by enriching dissociated cells using fluorescent sorting. However, these cell samples often have low yields of RNA that present challenges in library preparation....

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Detalles Bibliográficos
Autores principales: Barrett, Alec, McWhirter, Rebecca, Taylor, Seth R, Weinreb, Alexis, Miller, David M, Hammarlund, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495925/
https://www.ncbi.nlm.nih.gov/pubmed/33856427
http://dx.doi.org/10.1093/g3journal/jkab121