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A head-to-head comparison of ribodepletion and polyA selection approaches for Caenorhabditis elegans low input RNA-sequencing libraries
A recent and powerful technique is to obtain transcriptomes from rare cell populations, such as single neurons in Caenorhabditis elegans, by enriching dissociated cells using fluorescent sorting. However, these cell samples often have low yields of RNA that present challenges in library preparation....
Autores principales: | Barrett, Alec, McWhirter, Rebecca, Taylor, Seth R, Weinreb, Alexis, Miller, David M, Hammarlund, Marc |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495925/ https://www.ncbi.nlm.nih.gov/pubmed/33856427 http://dx.doi.org/10.1093/g3journal/jkab121 |
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