A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

Alkyne functional groups have Raman signatures in a region (1800 cm(−1) to 2800 cm(−1)) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue...

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Detalles Bibliográficos
Autores principales: Bakthavatsalam, Subha, Dodo, Kosuke, Sodeoka, Mikiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496067/
https://www.ncbi.nlm.nih.gov/pubmed/34704046
http://dx.doi.org/10.1039/d1cb00116g
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author Bakthavatsalam, Subha
Dodo, Kosuke
Sodeoka, Mikiko
author_facet Bakthavatsalam, Subha
Dodo, Kosuke
Sodeoka, Mikiko
author_sort Bakthavatsalam, Subha
collection PubMed
description Alkyne functional groups have Raman signatures in a region (1800 cm(−1) to 2800 cm(−1)) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique.
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spelling pubmed-84960672021-10-25 A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems Bakthavatsalam, Subha Dodo, Kosuke Sodeoka, Mikiko RSC Chem Biol Chemistry Alkyne functional groups have Raman signatures in a region (1800 cm(−1) to 2800 cm(−1)) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique. RSC 2021-07-14 /pmc/articles/PMC8496067/ /pubmed/34704046 http://dx.doi.org/10.1039/d1cb00116g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Bakthavatsalam, Subha
Dodo, Kosuke
Sodeoka, Mikiko
A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title_full A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title_fullStr A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title_full_unstemmed A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title_short A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems
title_sort decade of alkyne-tag raman imaging (atri): applications in biological systems
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496067/
https://www.ncbi.nlm.nih.gov/pubmed/34704046
http://dx.doi.org/10.1039/d1cb00116g
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