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Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom

Botrytis cinerea is a fungal pathogen that causes necrotic disease on more than a thousand known hosts widely spread across the plant kingdom. How B. cinerea interacts with such extensive host diversity remains largely unknown. To address this question, we generated an infectivity matrix of 98 strai...

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Autores principales: Caseys, Celine, Shi, Gongjun, Soltis, Nicole, Gwinner, Raoni, Corwin, Jason, Atwell, Susanna, Kliebenstein, Daniel J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496218/
https://www.ncbi.nlm.nih.gov/pubmed/34003931
http://dx.doi.org/10.1093/g3journal/jkab175
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author Caseys, Celine
Shi, Gongjun
Soltis, Nicole
Gwinner, Raoni
Corwin, Jason
Atwell, Susanna
Kliebenstein, Daniel J
author_facet Caseys, Celine
Shi, Gongjun
Soltis, Nicole
Gwinner, Raoni
Corwin, Jason
Atwell, Susanna
Kliebenstein, Daniel J
author_sort Caseys, Celine
collection PubMed
description Botrytis cinerea is a fungal pathogen that causes necrotic disease on more than a thousand known hosts widely spread across the plant kingdom. How B. cinerea interacts with such extensive host diversity remains largely unknown. To address this question, we generated an infectivity matrix of 98 strains of B. cinerea on 90 genotypes representing eight host plants. This experimental infectivity matrix revealed that the disease outcome is largely explained by variations in either the host resistance or pathogen virulence. However, the specific interactions between host and pathogen account for 16% of the disease outcome. Furthermore, the disease outcomes cluster among genotypes of a species but are independent of the relatedness between hosts. When analyzing the host specificity and virulence of B. cinerea, generalist strains are predominant. In this fungal necrotroph, specialization may happen by a loss in virulence on most hosts rather than an increase of virulence on a specific host. To uncover the genetic architecture of Botrytis host specificity and virulence, a genome-wide association study (GWAS) was performed and revealed up to 1492 genes of interest. The genetic architecture of these traits is widespread across the B. cinerea genome. The complexity of the disease outcome might be explained by hundreds of functionally diverse genes putatively involved in adjusting the infection to diverse hosts.
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spelling pubmed-84962182021-10-07 Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom Caseys, Celine Shi, Gongjun Soltis, Nicole Gwinner, Raoni Corwin, Jason Atwell, Susanna Kliebenstein, Daniel J G3 (Bethesda) Investigation Botrytis cinerea is a fungal pathogen that causes necrotic disease on more than a thousand known hosts widely spread across the plant kingdom. How B. cinerea interacts with such extensive host diversity remains largely unknown. To address this question, we generated an infectivity matrix of 98 strains of B. cinerea on 90 genotypes representing eight host plants. This experimental infectivity matrix revealed that the disease outcome is largely explained by variations in either the host resistance or pathogen virulence. However, the specific interactions between host and pathogen account for 16% of the disease outcome. Furthermore, the disease outcomes cluster among genotypes of a species but are independent of the relatedness between hosts. When analyzing the host specificity and virulence of B. cinerea, generalist strains are predominant. In this fungal necrotroph, specialization may happen by a loss in virulence on most hosts rather than an increase of virulence on a specific host. To uncover the genetic architecture of Botrytis host specificity and virulence, a genome-wide association study (GWAS) was performed and revealed up to 1492 genes of interest. The genetic architecture of these traits is widespread across the B. cinerea genome. The complexity of the disease outcome might be explained by hundreds of functionally diverse genes putatively involved in adjusting the infection to diverse hosts. Oxford University Press 2021-05-18 /pmc/articles/PMC8496218/ /pubmed/34003931 http://dx.doi.org/10.1093/g3journal/jkab175 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Investigation
Caseys, Celine
Shi, Gongjun
Soltis, Nicole
Gwinner, Raoni
Corwin, Jason
Atwell, Susanna
Kliebenstein, Daniel J
Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title_full Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title_fullStr Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title_full_unstemmed Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title_short Quantitative interactions: the disease outcome of Botrytis cinerea across the plant kingdom
title_sort quantitative interactions: the disease outcome of botrytis cinerea across the plant kingdom
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496218/
https://www.ncbi.nlm.nih.gov/pubmed/34003931
http://dx.doi.org/10.1093/g3journal/jkab175
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