Genome and transcriptome of a pathogenic yeast, Candida nivariensis

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50...

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Detalles Bibliográficos
Autores principales: Fan, Yunfan, Gale, Andrew N, Bailey, Anna, Barnes, Kali, Colotti, Kiersten, Mass, Michal, Morina, Luke B, Robertson, Bailey, Schwab, Remy, Tselepidakis, Niki, Timp, Winston
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496292/
https://www.ncbi.nlm.nih.gov/pubmed/33890630
http://dx.doi.org/10.1093/g3journal/jkab137
Descripción
Sumario:We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

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