Genome and transcriptome of a pathogenic yeast, Candida nivariensis

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50...

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Detalles Bibliográficos
Autores principales: Fan, Yunfan, Gale, Andrew N, Bailey, Anna, Barnes, Kali, Colotti, Kiersten, Mass, Michal, Morina, Luke B, Robertson, Bailey, Schwab, Remy, Tselepidakis, Niki, Timp, Winston
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Investigation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496292/
https://www.ncbi.nlm.nih.gov/pubmed/33890630
http://dx.doi.org/10.1093/g3journal/jkab137
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author Fan, Yunfan
Gale, Andrew N
Bailey, Anna
Barnes, Kali
Colotti, Kiersten
Mass, Michal
Morina, Luke B
Robertson, Bailey
Schwab, Remy
Tselepidakis, Niki
Timp, Winston
author_facet Fan, Yunfan
Gale, Andrew N
Bailey, Anna
Barnes, Kali
Colotti, Kiersten
Mass, Michal
Morina, Luke B
Robertson, Bailey
Schwab, Remy
Tselepidakis, Niki
Timp, Winston
author_sort Fan, Yunfan
collection PubMed
description We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.
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spelling pubmed-84962922021-10-07 Genome and transcriptome of a pathogenic yeast, Candida nivariensis Fan, Yunfan Gale, Andrew N Bailey, Anna Barnes, Kali Colotti, Kiersten Mass, Michal Morina, Luke B Robertson, Bailey Schwab, Remy Tselepidakis, Niki Timp, Winston G3 (Bethesda) Investigation We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata. Oxford University Press 2021-04-23 /pmc/articles/PMC8496292/ /pubmed/33890630 http://dx.doi.org/10.1093/g3journal/jkab137 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Fan, Yunfan
Gale, Andrew N
Bailey, Anna
Barnes, Kali
Colotti, Kiersten
Mass, Michal
Morina, Luke B
Robertson, Bailey
Schwab, Remy
Tselepidakis, Niki
Timp, Winston
Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title_full Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title_fullStr Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title_full_unstemmed Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title_short Genome and transcriptome of a pathogenic yeast, Candida nivariensis
title_sort genome and transcriptome of a pathogenic yeast, candida nivariensis
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8496292/
https://www.ncbi.nlm.nih.gov/pubmed/33890630
http://dx.doi.org/10.1093/g3journal/jkab137
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